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Literature summary for 3.4.21.5 extracted from
- Rapid kinetics of Na+ binding to thrombin (2006), J. Biol. Chem., 281, 40049-40056.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of enzyme mutants in BHK cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | stopped flow kinetics of Na+ binding to thrombin, overview | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | kinetic mechanism of Na+ binding to thrombin, stopped-flow measurements of intrinsic fluorescence, two-step mechanism with a rapid phase occurring within the dead time of the spectrometer followed by a single-exponential slow phase whose kobs decreases hyperbolically with increasing Na+ concentration, overview | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | wild-type and mutant enzyme activation by the prothrombinase complex | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme mutants from BHK cells by anion and cation exchange chromatography to homogeneity | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 25 | assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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