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Literature summary for 3.4.21.49 extracted from
- Specificity of the collagenase from the insect Hypoderma lineatum (1985), Eur. J. Biochem., 152, 123-130.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hypoderma lineatum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Hypoderma lineatum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | the first cleavage probably occurs between residues 764 and 765 at Pro-Ala and Thr-Ala bonds on the alpha1 and alpha2 chains respectively. Two more bonds would then by cleaved on the alpha1 chain, Ile776-Ala777 and Val782-Val783, and one more on the alpha2-chain, Ser800-Val801 | Hypoderma lineatum | ? | - |
? |  |
| oxidized insulin B chain + H2O | major cleavage at Arg22-Gly23 and Lys29-Ala30 bonds | Hypoderma lineatum | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
2 h, stable below | Hypoderma lineatum |
| 75 | - |
20 min, 55% loss of activity. 2 h, complete inactivation | Hypoderma lineatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | hydrolysis of collagen | Hypoderma lineatum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 30% of maximal activity, pH 10.0: about 80% of maximal activity, hydrolysis of collagen | Hypoderma lineatum |
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Release 2023.1 (January 2023)
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