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Literature summary for 3.4.21.48 extracted from
- Purification and properties of proteinase B from yeast (1981), Biochim. Biophys. Acta, 661, 124-135.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing in absence of glycerol results in total loss of activity | Saccharomyces cerevisiae |
| HgCl2, 10 mM, stabilizes during purification | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Saccharomyces cerevisiae |  |
| Aprotinin | i.e. trasylol, weak | Saccharomyces cerevisiae | |
| chymostatin | - |
Saccharomyces cerevisiae | |
| DFP | - |
Saccharomyces cerevisiae | |
| Hg2+ | HgCl2 | Saccharomyces cerevisiae | |
| p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
| PMSF | - |
Saccharomyces cerevisiae | |
| Trypsin inhibitor | weak | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Saccharomyces cerevisiae |
| 33700 | - |
equilibroum sedimentation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
Saccharomyces carlsbergensis | - |
| Saccharomyces cerevisiae | - |
Saccharomyces cerevisiae | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | contains 8-9% neutral sugars and 1.5% amino sugars | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| large scale | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, 0.1 mM HgCl2, stable for 2 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Azocoll + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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