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Literature summary for 3.4.21.48 extracted from

  • Kato, M.; Kuzuhara, Y.; Maeda, H.; Shiraga, S.; Ueda, M.
    Analysis of a processing system for proteases using yeast cell surface engineering: conversion of precursor of proteinase A to active proteinase A (2006), Appl. Microbiol. Biotechnol., 72, 1229-1237.
    View publication on PubMed
Search for more literature for 3.4.21.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5-alpha, generation and transformation of protease B-knockout strain of Saccaromyces termed MT8-1/delta-PrB described Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole vacuolar proenzyme Saccharomyces cerevisiae 5773
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 cerevisin precursor, vacuolar protease B, encoded by the PRB1 gene; after disruption of proteinase B termed MT8-1/delta-PrB
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Saccharomyces cerevisiae MT8-1
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 cerevisin precursor, vacuolar protease B, encoded by the PRB1 gene; after disruption of proteinase B termed MT8-1/delta-PrB
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 processing of different vacuolar proteases by using protease B-knockout strains indicated Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information protease-knockout strains constructed, processing of vacuolar proteases using a novel cell-surface engineering technique described Saccharomyces cerevisiae ?
-
 ?
additional information protease-knockout strains constructed, processing of vacuolar proteases using a novel cell-surface engineering technique described Saccharomyces cerevisiae MT8-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Proteinase B
-
 Saccharomyces cerevisiae