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Literature summary for 3.4.21.47 extracted from
- The cobra venom factor-dependent C3 convertase of human complement. A kinetic and thermodynamic analysis of a protease acting on its natural high molecular weight substrate (1982), J. Biol. Chem., 257, 8292-8299.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0116 | - |
complement component C3 | - |
Homo sapiens | |
| 0.0144 | - |
complement component C3 | - |
Homo sapiens | |
| 0.0176 | - |
complement component C3 | - |
Homo sapiens | |
| 0.0264 | - |
complement component C3 | - |
Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Cleavage of Arg-/-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-/- bond in complement component C5 alpha-chain to yield C5a and C5b | the enzyme is a bimolecular complex of complement fragment Bb with either C3b or cobra venom factor. Bb contains the active site. Bb is formed by cleavage of proenzyme factor B by factor D. Cleavage of complement component C5 requires additional C3b which binds C5 and renders it susceptible to cleavage by C3b,Bb complex, , intermediate catalytic mechanism and a kinetic mechanism of a tetra uni ping pong bi bi reaction reduced to a uni bi reaction | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C3 + H2O | hydrolysis of peptide bond 77 Arg-Ser of the a-chain | Homo sapiens | complement component C3a + complement component C3b | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
spontaneous decay-dissociation into its subunits with a half-life of 7 h | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.107 | - |
complement component C3 | - |
Homo sapiens | |
| 0.202 | - |
complement component C3 | - |
Homo sapiens | |
| 0.367 | - |
complement component C3 | - |
Homo sapiens | |
| 0.472 | - |
complement component C3 | - |
Homo sapiens |
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