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Literature summary for 3.4.21.46 extracted from
- Crystal structure of a complement factor D mutant expressing enhanced catalytic activity [published erratum appears in J Biol Chem 1995 Dec 29;270(52):31414 (1995), J. Biol. Chem., 270, 24399-24405.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| wild type and mutant factor D, expression in CHO cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S94Y | 6fold increase in turnover number | Homo sapiens |
| S94Y/T214/S215W | the triple mutant has a turnover number that is 16fold higher than that of wild type factor D | Homo sapiens |
| T214S/S215W | more than 16fold incerease of the ratio of turnover number/KM-value | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.95 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y/T214S/S215W | Homo sapiens |  |
| 3.3 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y/T214S | Homo sapiens | |
| 3.79 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | wild-type enzyme | Homo sapiens | |
| 4.72 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y/S214W | Homo sapiens | |
| 5.15 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y | Homo sapiens | |
| 5.64 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme T214S | Homo sapiens | |
| 6.37 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme T214S/S215W | Homo sapiens | |
| 11.44 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S215W | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Lys thiobenzyl ester + H2O | - |
Homo sapiens | benzyloxycarbonyl-Lys + phenylmethanethiol | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | wild-type enzyme | Homo sapiens | |
| 1.84 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme T214S | Homo sapiens | |
| 4.5 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S215W | Homo sapiens | |
| 6.31 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y/S214W | Homo sapiens | |
| 7.06 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme T214S/S215W | Homo sapiens | |
| 7.45 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y | Homo sapiens | |
| 12 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y/T214S | Homo sapiens | |
| 21.3 | - |
Benzyloxycarbonyl-Lys-thiobenzyl ester | mutant enzyme S94Y/T214S/S215W | Homo sapiens | |
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