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Literature summary for 3.4.21.42 extracted from

  • Skoog, S.M.; Wiseman, J.S.; Bey, P.
    The specificity of two proteinases that cleave adjacent to argirnine, Cl esterase and acrosin, for pepfide p-nitroanilide substrates (1989), Biochim. Biophys. Acta, 996, 89.94 .
    View publication on PubMed
Search for more literature for 3.4.21.42

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Vmax/KM values, kinetics with peptide 4-nitroanilide substrates, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09871
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification inactive precursor, Cls, and fully activated proteinase, Clg Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-beta-Ala-Gly-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzoyl-beta-Ala-Gly-L-Arg + 4-nitroaniline
-
 ?
benzoyl-L-Pro-L-Phe-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
-
 ?
benzoyl-L-Val-Gly-L-Arg 4-nitroaniline + H2O
-
 Homo sapiens benzoyl-L-Val-Gly-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Gly-D-Ala-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-D-Ala-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Gly-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-L-Pro-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Arg-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Arg-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Lys 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Lys + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Lys-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Lys-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Phe-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Tyr-L-Lys-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Tyr-L-Lys-L-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-L-Val-Gly-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Val-Gly-L-Arg + 4-nitroaniline
-
 ?
butyloxycarbonyl-L-Leu-Gly-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens butyloxycarbonyl-L-Leu-Gly-L-Arg + 4-nitroaniline
-
 ?
butyloxycarbonyl-O-benzoyl-L-Ser-Gly-Arg 4-nitroanilide + H2O
-
 Homo sapiens butyloxycarbonyl-O-benzoyl-L-Ser-Gly-Arg + 4-nitroaniline
-
 ?
D-CHG-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens D-CHG-L-Pro-L-Arg + 4-nitroaniline
-
 ?
D-Ile-L-Phe-L-Lys 4-nitroanilide + H2O
-
 Homo sapiens D-Ile-L-Phe-L-Lys + 4-nitroaniline
-
 ?
D-Ile-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens D-Ile-L-Pro-L-Arg + 4-nitroaniline
-
 ?
D-Pro-L-hexahydrotyrosine-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens D-Pro-L-hexahydrotyrosine-L-Arg + 4-nitroaniline
-
 ?
D-Pro-L-Phe-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens D-Pro-L-Phe-L-Arg + 4-nitroaniline
-
 ?
D-Val-L-cyclohexylalanine-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens D-Val-L-cyclohexylalanine-L-Arg + 4-nitroaniline
-
 ?
D-Val-L-Leu-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens D-Val-L-Leu-L-Arg + 4-nitroaniline
-
 ?
D-Val-L-Leu-L-Lys 4-nitroanilide + H2O
-
 Homo sapiens D-Val-L-Leu-L-Lys + 4-nitroaniline
-
 ?
D-Val-L-Phe-L-Lys 4-nitroanilide + H2O
-
 Homo sapiens D-Val-L-Phe-L-Lys + 4-nitroaniline
-
 ?
Gly-Arg 4-nitroanilide + H2O
-
 Homo sapiens Gly-Arg + 4-nitroaniline
-
 ?
L-Glu-Gly-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens L-Glu-Gly-L-Arg + 4-nitroaniline
-
 ?
L-Glu-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens L-Glu-L-Pro-L-Arg + 4-nitroaniline
-
 ?
methyloxycarbonyl-D-L-hexahydrotyrosine-Gly-L-Arg 4-nitroaniline + H2O
-
 Homo sapiens methyloxycarbonyl-D-L-hexahydrotyrosine-Gly-L-Arg + 4-nitroaniline
-
 ?
methyloxycarbonyl-D-Nle-Gly-L-Arg 4-nitroaniline + H2O
-
 Homo sapiens methyloxycarbonyl-D-Nle-Gly-L-Arg + 4-nitroaniline
-
 ?
methyloxycarbonyl-L-cyclohexylglycine-Gly-L-Arg 4-nitroaniline + H2O
-
 Homo sapiens methyloxycarbonyl-L-cyclohexylglycine-Gly-L-Arg + 4-nitroaniline
-
 ?
additional information the enzyme cleaves after Arg-/- or Lys-/- and prefers arginine at over 3fold over lysine, the enzyme does not accept citrulline. Occupancy of site S3 may yield an increase in Vmax/Km of over 10fold with the enzyme, but many residues are accepted at positions S2, S3 and S4. No activity with benzyloxycarbonyl-Gly-L-Pro-citrulline 4-nitroanilide Homo sapiens ?
-
 ?
N2-acetyl-L-Lys-N6-benzyloxycarbonyl-Gly-L-Arg 4-nitroanilide + H2O best substrate Homo sapiens N2-acetyl-L-Lys-N6-benzyloxycarbonyl-Gly-L-Arg + 4-nitroaniline
-
 ?
N2-acetyl-N6-benzyloxycarbonyl-L-Lys-Gly-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens N2-acetyl-N6-benzyloxycarbonyl-L-Lys-Gly-L-Arg + 4-nitroaniline
-
 ?
N6-benzyloxycarbonyl-D-Lys-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens N6-benzyloxycarbonyl-D-Lys-L-Pro-L-Arg + 4-nitroaniline
-
 ?
Sar-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens Sar-L-Pro-L-Arg + 4-nitroaniline
-
 ?
tosyl-Gly-L-Pro-L-Arg 4-nitroanilide + H2O
-
 Homo sapiens tosyl-Gly-L-Pro-L-Arg + 4-nitroaniline
-
 ?
tosyl-Gly-L-Pro-L-Lys 4-nitroanilide + H2O
-
 Homo sapiens tosyl-Gly-L-Pro-L-Lys + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
C1 esterase
-
 Homo sapiens
C1g
-
 Homo sapiens
C1s
-
 Homo sapiens
complement component 1 subcomponent s UniProt Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function Cls is the second enzyme in the cascade of complement activation. This enzyme can be obtained as its inactive precursor, Cls, as the fully activated proteinase, Clg, or as a subunit of the first component of complement, Cl. In addition to Clg, CI contains two other proteins: Clq and a second proteinase, Clr. Activation of Cl by immune complexes, by freeze/thaw or by incubation with calcium plus lipopolysaccharide results first in conversion of inactive Cls to the proteinase Clr Homo sapiens