Cloned (Comment) | Organism |
---|---|
development and evaluation of a method for expression of recombinant full-length human C-terminally tagged C1q involving stable transfection of HEK 293-F mammalian cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement C1q zymogen + H2O | Homo sapiens | - |
active complement C1q + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09871 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement C1q zymogen + H2O | - |
Homo sapiens | active complement C1q + ? | - |
? | |
complement C1q zymogen + H2O | no or reduced activity with substrate mutants K59A, K61A, K58A, and K58A/K59A/K61A | Homo sapiens | active complement C1q + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protease C1s | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | hexameric complement C1q is a versatile recognition protein that senses a wide variety of immune and nonimmune ligands, including pathogens and altered self components, and triggers the classical complement pathway through activation of its associated proteases C1r, EC 3.4.21.41, and C1s. Residues LysB61 and LysC58 each play a key role in the interaction with C1s-C1r-C1r-C1s, with LysA59 being involved to a lesser degree | Homo sapiens |