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Literature summary for 3.4.21.42 extracted from

  • Bally, I.; Rossi, V.; Thielens, N.M.; Gaboriaud, C.; Arlaud, G.J.
    Functional role of the linker between the complement control protein modules of complement protease C1s (2005), J. Immunol., 175, 4536-4542.
    View publication on PubMed
Search for more literature for 3.4.21.42

Cloned(Commentary)

Cloned (Comment) Organism
expression in a baculovirus/insect cells system Homo sapiens

Protein Variants

Protein Variants Comment Organism
D343N complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
P341I complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340A similar to wild-type Homo sapiens
Q340D complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340D/D343E complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340E activation by complement component C1r is impaired, enhanced glycosylation at N391 Homo sapiens
Q340E/D343N complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340E/N391Q similar to wild-type Homo sapiens
Q340K complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340K/D343N complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340K/P341I complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340K/P341I/V342K complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340R complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340S complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
Q340S/P341I complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens
V342K complement component C4 cleavage activity below 50% compared to wild-type, complement component C2 cleavage activity similar to wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 Complement component C4 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77410
-
 mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
one-step affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C2 + H2O
-
 Homo sapiens complement component C2a + complement component C2b
-
 ?
complement component C4 + H2O
-
 Homo sapiens complement component C4a + complement component C4b
-
 ?

Synonyms

Synonyms Comment Organism
complement protease C1s
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.7
-
 Complement component C4 145 mM NaCl, 50 mM triethanolamine hydrochloride, 1 mM CaCl2, 1 mg/ml ovalbumin pH 7.4, 37°C Homo sapiens