Any feedback?
Literature summary for 3.4.21.42 extracted from
- Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates (2003), Biochemistry, 42, 14939-14945.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | K0.5-values | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(dinitrophenyl)-NH2 + H2O | - |
Homo sapiens | 2-aminobenzoyl-Gly-Leu-Gln-Arg + Ala-Leu-Glu-Ile-Lys(dinitrophenyl)-NH2 | - |
? | |
| 2-aminobenzoyl-Ser-Leu-Gly-Arg-Lys-Ile-Gln-Ile-Lys(dinitrophenyl)-NH2 + H2O | - |
Homo sapiens | 2-aminobenzoyl-Ser-Leu-Gly-Arg + Lys-Ile-Gln-Ile-Lys(dinitrophenyl)-NH2 | - |
? | |
| benzyloxycarbonyl-Gly-Leu-Gln-Arg-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Leu-Gln-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Ser-Leu-Gly-Arg-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Ser-Leu-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
| complement component C2 + H2O | the P1'-P4' substrate residues of C2 confer greater affinity of substrate for enzyme and also induce a sigmoidal dependence of enzyme velocity on substrate concentration. The substrate gives rise to homotropic positive cooperative behavior in the enzyme | Homo sapiens | complement component C2a + complement component C2b | - |
? | |
| complement component C4 + H2O | the P1'-P4' substrate residues of C4 confer greater affinity of substrate for enzyme and also induce a sigmoidal dependence of enzyme velocity on substrate concentration. The substrate gives rise to homotropic positive cooperative behavior in the enzyme | Homo sapiens | complement component C4a + complement component C4b | - |
? | |
| GLQRA + H2O | - |
Homo sapiens | GLQR + Ala | - |
? | |
| GLQRAAEI + H2O | - |
Homo sapiens | GLQR + AAEI | - |
? | |
| GLQRAEEI + H2O | - |
Homo sapiens | GLQR + AEEI | - |
? | |
| GLQRAFEI + H2O | - |
Homo sapiens | GLQR + AFEI | - |
? | |
| GLQRAHEI + H2O | - |
Homo sapiens | GLQR + AHEI | - |
? | |
| GLQRAL + H2O | - |
Homo sapiens | GLQR + Ala-Leu | - |
? | |
| GLQRALAI + H2O | - |
Homo sapiens | GLQR + ALAI | - |
? | |
| GLQRALE + H2O | - |
Homo sapiens | GLQR + ALE | - |
? | |
| GLQRALEA + H2O | - |
Homo sapiens | GLQR + ALEA | - |
? | |
| GLQRALEE + H2O | - |
Homo sapiens | GLQR + ALEE | - |
? | |
| GLQRALEF + H2O | - |
Homo sapiens | GLQR + ALEF | - |
? | |
| GLQRALEH + H2O | - |
Homo sapiens | GLQR + ALEH | - |
? | |
| GLQRALEI + H2O | - |
Homo sapiens | GLQR + ALEI | - |
? | |
| GLQRALEK + H2O | - |
Homo sapiens | GLQR + ALEK | - |
? | |
| GLQRALEL + H2O | - |
Homo sapiens | GLQR + ALEL | - |
? | |
| GLQRALEM + H2O | - |
Homo sapiens | GLQR + ALEM | - |
? | |
| GLQRALEP + H2O | - |
Homo sapiens | GLQR + ALEP | - |
? | |
| GLQRALEQ + H2O | - |
Homo sapiens | GLQR + ALEQ | - |
? | |
| GLQRALET + H2O | - |
Homo sapiens | GLQR + ALET | - |
? | |
| GLQRALFI + H2O | - |
Homo sapiens | GLQR + ALFI | - |
? | |
| GLQRALHI + H2O | - |
Homo sapiens | GLQR + ALHI | - |
? | |
| GLQRALKI + H2O | - |
Homo sapiens | GLQR + ALKI | - |
? | |
| GLQRALLI + H2O | - |
Homo sapiens | GLQR + ALLI | - |
? | |
| GLQRALMI + H2O | - |
Homo sapiens | GLQR + ALMI | - |
? | |
| GLQRALPI + H2O | - |
Homo sapiens | GLQR + ALPI | - |
? | |
| GLQRALQI + H2O | - |
Homo sapiens | GLQR + ALQI | - |
? | |
| GLQRALTI + H2O | - |
Homo sapiens | GLQR + ALTI | - |
? | |
| GLQRAMEI + H2O | - |
Homo sapiens | GLQR + AMEI | - |
? | |
| GLQRAPEI + H2O | - |
Homo sapiens | GLQR + APEI | - |
? | |
| GLQRAQEI + H2O | - |
Homo sapiens | GLQR + AQEI | - |
? | |
| GLQRATEI + H2O | - |
Homo sapiens | GLQR + ATEI | - |
? | |
| GLQRELEI + H2O | - |
Homo sapiens | GLQR + ELEI | - |
? | |
| GLQRFLEI + H2O | - |
Homo sapiens | GLQR + FLEI | - |
? | |
| GLQRHLEI + H2O | - |
Homo sapiens | GLQR + HLEI | - |
? | |
| GLQRKLEI + H2O | - |
Homo sapiens | GLQR + KLEI | - |
? | |
| GLQRLLEI + H2O | - |
Homo sapiens | GLQR + LLEI | - |
? | |
| GLQRMLEI + H2O | - |
Homo sapiens | GLQR + MLEI | - |
? | |
| GLQRQLEI + H2O | - |
Homo sapiens | GLQR + QLEI | - |
? | |
| GLQRTLEI + H2O | - |
Homo sapiens | GLQR + TLEI | - |
? | |
| additional information | importance of the prime subsites of the C1s protease for recognition of substrates no cleavage of GLQRPLEI and GLQRAKEI | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000189 | - |
GLQRAPEI | pH 7.4, 37°C | Homo sapiens | |
| 0.0000205 | - |
GLQRELEI | pH 7.4, 37°C | Homo sapiens | |
| 0.0000394 | - |
GLQRA | pH 7.4, 37°C | Homo sapiens | |
| 0.0000421 | - |
GLQRAEEI | pH 7.4, 37°C | Homo sapiens | |
| 0.0000702 | - |
GLQRLLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.0000702 | - |
GLQRQLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.0000762 | - |
GLQRKLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.0000978 | - |
GLQRALEL | pH 7.4, 37°C | Homo sapiens | |
| 0.0000978 | - |
GLQRALEQ | pH 7.4, 37°C | Homo sapiens | |
| 0.0000978 | - |
GLQRALEE | pH 7.4, 37°C | Homo sapiens | |
| 0.000119 | - |
GLQRAMEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000136 | - |
GLQRALKI | pH 7.4, 37°C | Homo sapiens | |
| 0.000147 | - |
GLQRATEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000155 | - |
GLQRFLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000169 | - |
GLQRAAEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000171 | - |
GLQRALEM | pH 7.4, 37°C | Homo sapiens | |
| 0.000183 | - |
GLQRAQEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000205 | - |
GLQRALEI | pH 7.4, 37°C | Homo sapiens | |
| 0.00021 | - |
2-aminobenzoyl-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(dinitrophenyl)-NH2 | pH 7.4, 37°C | Homo sapiens | |
| 0.00022 | - |
GLQRALEF | pH 7.4, 37°C | Homo sapiens | |
| 0.000239 | - |
GLQRMLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000244 | - |
GLQRALEH | pH 7.4, 37°C | Homo sapiens | |
| 0.000319 | - |
GLQRALE | pH 7.4, 37°C | Homo sapiens | |
| 0.000323 | - |
GLQRAQEI | pH 7.4, 37°C | Homo sapiens | |
| 0.00033 | - |
GLQRAFEI | pH 7.4, 37°C | Homo sapiens | |
| 0.000392 | - |
GLQRALEA | pH 7.4, 37°C | Homo sapiens | |
| 0.000415 | - |
GLQRHLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.00044 | - |
GLQRALET | pH 7.4, 37°C | Homo sapiens | |
| 0.000683 | - |
GLQRAL | pH 7.4, 37°C | Homo sapiens | |
| 0.000807 | - |
GLQRALEP | pH 7.4, 37°C | Homo sapiens | |
| 0.000867 | - |
GLQRALEK | pH 7.4, 37°C | Homo sapiens | |
| 0.00139 | - |
2-aminobenzoyl -Ser-Leu-Gly-Arg-Lys-Ile-Gln-Ile-Lys(dinitrophenyl)-NH2 | pH 7.4, 37°C | Homo sapiens | |
| 0.00155 | - |
GLQRTLEI | pH 7.4, 37°C | Homo sapiens | |
| 0.00227 | - |
GLQRALMI | pH 7.4, 37°C | Homo sapiens | |
| 0.00232 | - |
GLQRAQEI | pH 7.4, 37°C | Homo sapiens | |
| 0.00244 | - |
GLQRALPI | pH 7.4, 37°C | Homo sapiens | |
| 0.00274 | - |
GLQRALAI | pH 7.4, 37°C | Homo sapiens | |
| 0.00279 | - |
GLQRALTI | pH 7.4, 37°C | Homo sapiens | |
| 0.00288 | - |
GLQRALFI | pH 7.4, 37°C | Homo sapiens | |
| 0.00296 | - |
GLQRALLI | pH 7.4, 37°C | Homo sapiens | |
| 0.00821 | - |
GLQRALHI | pH 7.4, 37°C | Homo sapiens | |
| 0.38 | - |
benzyloxycarbonyl-Gly-Leu-Gln-Arg-4-methylcoumaryl-7-amide | pH 7.4, 37°C | Homo sapiens | |
| 0.5 | - |
benzyloxycarbonyl-Ser-Leu-Gly-Arg-4-methylcoumaryl-7-amide | pH 7.4, 37°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
