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Literature summary for 3.4.21.4 extracted from
- Trypsin from the digestive system of carp Cirrhinus mrigala: purification, characterization and its potential application (2015), Food Chem., 175, 386-394.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 10 mM, 48% inhibition | Cirrhinus mrigala |  |
| Co2+ | 10 mM, 31.6% inhibition | Cirrhinus mrigala | |
| Hg2+ | 10 mM, 85.7% inhibition | Cirrhinus mrigala | |
| K+ | 10 mM, 16% inhibition | Cirrhinus mrigala | |
| Li+ | 10 mM, 19% inhibition | Cirrhinus mrigala | |
| Mg2+ | 10 mM, 21.6% inhibition | Cirrhinus mrigala | |
| N-alpha-p-tosyl-L-lysine chloromethyl ketone | 10 mM, 99.67% inhibition | Cirrhinus mrigala | |
| Na+ | 10 mM, 21% inhibition | Cirrhinus mrigala | |
| phenylmethylsulfonyl fluoride | 100 mM, complete inhibition | Cirrhinus mrigala | |
| Soybean trypsin inhibitor | 250 mM, complete inhibition | Cirrhinus mrigala | |
| Zn2+ | 10 mM, 31.6% inhibition | Cirrhinus mrigala | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
benzoyl-DL-Arg-4-nitroanilide | pH 7.6, 25°C | Cirrhinus mrigala | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21700 | - |
x * 21700, SDs-PAGE | Cirrhinus mrigala |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cirrhinus mrigala | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.07 | - |
pH 7.6, 25°C | Cirrhinus mrigala |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-DL-Arg-4-nitroanilide + H2O | - |
Cirrhinus mrigala | benzoyl-DL-Arg + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21700, SDs-PAGE | Cirrhinus mrigala |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | - |
Cirrhinus mrigala |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for 1 h, in presence of 2 mM Ca2+ stable for upto 7 h | Cirrhinus mrigala |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.17 | - |
benzoyl-DL-Arg-4-nitroanilide | pH 7.6, 25°C | Cirrhinus mrigala | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Cirrhinus mrigala |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.2 | - |
Cirrhinus mrigala |
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