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Literature summary for 3.4.21.4 extracted from

  • Toth, J.; Simon, Z.; Medveczky, P.; Gombos, L.; Jelinek, B.; Szilagyi, L.; Graf, L.; Malnasi-Csizmadia, A.
    Site directed mutagenesis at position 193 of human trypsin 4 alters the rate of conformational change during activation: role of local internal viscosity in protein dynamics (2007), Proteins, 67, 1119-1127.
    View publication on PubMed
Search for more literature for 3.4.21.4

Protein Variants

Protein Variants Comment Organism
R193A the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation Homo sapiens
R193F the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation Homo sapiens
R193G the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation Homo sapiens
R193Y the mutation does not affect the activation energy of the reaction, but significantly alters the preexponential term of the Arrhenius equation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0135
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
0.0198
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
0.0213
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
0.0252
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
0.0274
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
-
 Homo sapiens N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
trypsin 4
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
110
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
124
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
133
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
158
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens
162
-
 N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C Homo sapiens