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Literature summary for 3.4.21.4 extracted from

  • Villalonga, M.L.; Reyes, G.; Fragoso, A.; Cao, R.; Fernandez, L.; Villalonga, R.
    Chemical glycosidation of trypsin with O-carboxymethyl-poly-beta-cyclodextrin: catalytic and stability properties (2005), Biotechnol. Appl. Biochem., 41, 217-223.
    View publication on PubMed
Search for more literature for 3.4.21.4

Application

Application Comment Organism
synthesis attachment of O-carboxymethyl-poly-beta-cyclodextrin with molecular weight of 13000 Da to surface of enzyme. resulting neoglycoenzyme retains high proteolytic and esterolytic activity, optimum temperature is increased by 10°C, enzyme is more resistant to thermal inactivation and to denaturation by sodiumdodecyl sulfate Bos taurus

Protein Variants

Protein Variants Comment Organism
additional information attachment of O-carboxymethyl-poly-beta-cyclodextrin with molecular weight of 13000 Da to surface of enzyme. Resulting neoglycoenzyme retains high proteolytic and esterolytic activity, optimum temperature is increased by 10°C, enzyme is more resistant to thermal inactivation and to denaturation by sodiumdodecyl sulfate Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32
-
 N-alpha-benzoyl-L-Arg ethyl ester enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25°C Bos taurus
35.5
-
 N-alpha-benzoyl-L-Arg ethyl ester wild-type, pH 8.0, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-Arg ethyl ester + H2O
-
 Bos taurus N-alpha-benzoyl-L-Arg + ethanol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 wild-type, half-life 58 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 187 min Bos taurus
50
-
 wild-type, half-life 16 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 97 min. Presence of 1 M NaCl, wild-type, half-life 41 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 48 min. Presence of 0.1 mM adamantan-1-ol, wild-type, half-life 14 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 29 min Bos taurus
60
-
 wild-type, half-life 6 min, enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, half-life 28 min Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.5
-
 N-alpha-benzoyl-L-Arg ethyl ester enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25°C Bos taurus
12.3
-
 N-alpha-benzoyl-L-Arg ethyl ester wild-type, pH 8.0, 25°C Bos taurus