Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.39 extracted from

  • Newlands, G.F.Y.; Knox, D.P.; Pirie-Sheperd, S.R.; Miller, H.R.P.
    Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4) (1993), Biochem. J., 294, 127-135.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.39

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin
-
 Mus musculus
Alpha1-proteinase inhibitor
-
 Mus musculus
diisopropyl fluorophosphate
-
 Mus musculus
phenylmethylsulfonyl fluoride
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.663
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1C Mus musculus
0.832
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1E Mus musculus
0.922
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1A Mus musculus
0.936
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1D Mus musculus
0.991
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1B Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 1 * 30000, enzyme form MMCP-1D and MMCP-1E, SDS-PAGE Mus musculus
31000
-
 1 * 31000, enzyme form MMCP-1B and MMCP-1C, SDS-PAGE Mus musculus
32000
-
 1 * 32000 + 1 * 33000, enzyme form MMCP-1A, SDS-PAGE Mus musculus
33000
-
 1 * 32000 + 1 * 33000, enzyme form MMCP-1A, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 5 enzyme forms: MMCP-1A to MMCP-1E
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification 5 variant glycoforms, differences in the electrophoretic mobility between the variant forms of MMCP-1 are probably a result of variable glycosylation Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
mast cell
-
 Mus musculus
-
small intestine
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg-4-nitroanilide + H2O no activity with enzyme forms 1D and 1E Mus musculus benzoyl-Arg + 4-nitroaniline
-
 ?
benzoyl-L-Tyr-4-nitroanilide + H2O activity with enzyme form 1A, no activity with enzyme form 1B, 1C, 1D, 1E Mus musculus benzoyl-L-Tyr + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl-Ala + 4-nitrophenyl
-
 ?
benzyloxycarbonyl-Arg-4-nitrophenyl ester + H2O no activity with enzyme form 1B, 1C and 1E Mus musculus benzyloxycarbonyl-Arg + 4-nitrophenol
-
 ?
benzyloxycarbonyl-D-Ala-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl + D-Ala-4-nitrophenol
-
 ?
benzyloxycarbonyl-Phe-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl-Phe + 4-nitrophenol
-
 ?
benzyloxycarbonyl-Trp-4-nitrophenyl ester + H2O
-
 Mus musculus benzyloxycarbonyl-Trp + 4-nitrophenol
-
 ?
benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O no activity with enzyme form 1C and 1E Mus musculus benzyloxycarbonyl-Tyr-4-nitrophenyl ester
-
 ?
succinyl-Phe-4-nitroanilide + H2O no activity with enzyme form 1C and 1D Mus musculus succinyl-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 32000 + 1 * 33000, enzyme form MMCP-1A, SDS-PAGE Mus musculus
monomer 1 * 30000, enzyme form MMCP-1D and MMCP-1E, SDS-PAGE Mus musculus
monomer 1 * 31000, enzyme form MMCP-1B and MMCP-1C, SDS-PAGE Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.25
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1E Mus musculus
1.66
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1B Mus musculus
2.33
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1A Mus musculus
2.54
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1D Mus musculus
2.84
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide enzyme form 1C Mus musculus