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Literature summary for 3.4.21.39 extracted from

  • Andersson, M.K.; Thorpe, M.; Hellman, L.
    Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2 of substrates (2010), FEBS J., 277, 2255-2267.
    View publication on PubMed
Search for more literature for 3.4.21.39

Cloned(Commentary)

Cloned (Comment) Organism
enterokinase cleavage site and N-terminal His6-tag, expressed in HEK 293 EBNA cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
K192M weakened preference for acidic amino acids at position P2' Homo sapiens
R143Q weakened preference for acidic amino acids at position P2' Homo sapiens
R143Q/K192M no preference for acidic amino acids at position P2' Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE (active enzyme) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), activation by His6-tag removal by cleavage with enterokinase, heparin-Sepharose chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
mast cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O
-
 Homo sapiens methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE (active enzyme) Homo sapiens

Synonyms

Synonyms Comment Organism
chymase
-
 Homo sapiens