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Literature summary for 3.4.21.39 extracted from

  • Caughey, G.H.; Beauchamp, J.; Schlatter, D.; Raymond, W.W.; Trivedi, N.N.; Banner, D.; Mauser, H.; Fingerle, J.
    Guinea pig chymase is leucine-specific: A novel example of functional plasticity in the chymase/granzyme family of serine peptidases (2008), J. Biol. Chem., 283, 13943-13951.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.39

Cloned(Commentary)

Cloned (Comment) Organism
mutant is expressed in Escherichia coli BL21(DE3) cells Cavia porcellus

Protein Variants

Protein Variants Comment Organism
A216G the partially humanized mutant acquires human-like P1 Phe- and angiotensin-cleaving capacity Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
alpha1-Aantichymotrypsin
-
 Cavia porcellus
alpha1-Aantichymotrypsin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme Homo sapiens
0.53
-
 Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme Cavia porcellus
0.65
-
 Ala-Ala-Pro-Phe-4-nitroanilide mutant enzyme A216G Cavia porcellus
0.97
-
 Ala-Ala-Pro-Met-4-nitroanilide wild type enzyme Cavia porcellus
1.02
-
 Ala-Ala-Pro-Leu-4-nitroanilide wild type enzyme Homo sapiens
1.5
-
 Ala-Ala-Pro-Leu-4-nitroanilide mutant enzyme A216G Cavia porcellus
1.55
-
 Ala-Ala-Pro-Leu-4-nitroanilide wild type enzyme Cavia porcellus
3
-
 Ala-Ala-Pro-Met-4-nitroanilide wild type enzyme Homo sapiens
27
-
 Ala-Ala-Pro-Met-4-nitroanilide mutant enzyme A216G Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus A7WPL7 precursor
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Cavia porcellus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Pro-Leu-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
Ala-Ala-Pro-Leu-4-nitroanilide + H2O
-
 Cavia porcellus Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
Ala-Ala-Pro-Met-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Pro-Met + 4-nitroaniline
-
 ?
Ala-Ala-Pro-Met-4-nitroanilide + H2O
-
 Cavia porcellus Ala-Ala-Pro-Met + 4-nitroaniline
-
 ?
Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
Ala-Ala-Pro-Phe-4-nitroanilide + H2O negligible activity Cavia porcellus Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
angiotensin I + H2O
-
 Homo sapiens angiotensin II + ?
-
 ?
additional information lacks elastase-like preference for P1 Val or Ala, and does not cleave angiotensin I Cavia porcellus ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-chymase
-
 Homo sapiens
alpha-chymase
-
 Cavia porcellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme Cavia porcellus
0.098
-
 Ala-Ala-Pro-Met-4-nitroanilide wild type enzyme Cavia porcellus
0.93
-
 Ala-Ala-Pro-Leu-4-nitroanilide wild type enzyme Cavia porcellus
3.9
-
 Ala-Ala-Pro-Leu-4-nitroanilide mutant enzyme A216G Cavia porcellus
13
-
 Ala-Ala-Pro-Met-4-nitroanilide mutant enzyme A216G Cavia porcellus
16
-
 Ala-Ala-Pro-Phe-4-nitroanilide mutant enzyme A216G Cavia porcellus
19.3
-
 Ala-Ala-Pro-Leu-4-nitroanilide wild type enzyme Homo sapiens
23
-
 Ala-Ala-Pro-Met-4-nitroanilide wild type enzyme Homo sapiens
143
-
 Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme Homo sapiens