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Literature summary for 3.4.21.39 extracted from

  • Lockhart, B.E.; Vencill, J.R.; Felix, C.M.; Johnson, D.A.
    Recombinant human mast-cell chymase: an improved procedure for expression in Pichia pastoris and purification of the highly active enzyme (2005), Biotechnol. Appl. Biochem., 41, 89-95.
    View publication on PubMed
Search for more literature for 3.4.21.39

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.96
-
 (25R)-3beta-hydroxycholest-5-en-27-oate pH 7.5, 22°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25030
-
 x * 30600, SDS-PAGE, x * 25030, calculated Homo sapiens
30600
-
 x * 30600, SDS-PAGE, x * 25030, calculated Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, procedure for expression and purification from culture media Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
mast cell recombinant enzyme Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(25R)-3beta-hydroxycholest-5-en-27-oate + H2O
-
 Homo sapiens ?
-
 ?
succinyl-AAPF-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Homo sapiens succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 30600, SDS-PAGE, x * 25030, calculated Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.5
-
 succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 22°C Homo sapiens