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Literature summary for 3.4.21.38 extracted from

  • Shan, J.; Baguinon, M.; Zheng, L.; Krishnamoorthi, R.
    Expression, refolding, and activation of the catalytic domain of human blood coagulation factor XII (2003), Protein Expr. Purif., 27, 143-149.
    View publication on PubMed
Search for more literature for 3.4.21.38

Cloned(Commentary)

Cloned (Comment) Organism
the cDNA of the zymogen domain of FXII is cloned into the pET-28a(+) vector and the plasmid is transformed into Escherichia coli strain BL21 (DE3) and overexpressed Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens increased levels of activated coagulation factor XIIa are associated with coronary heart disease, sepsis, and diabetes ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification contains a C-terminal serine protease zymogen domain, which becomes activated upon contacting a negative surface Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
the cDNA of the zymogen domain of FXII is cloned into the pET-28a(+) vector and the plasmid is transformed into Escherichia coli strain BL21 (DE3) and overexpressed. The multi-disulfide, recombinant zymogen domain His(6)-beta-FXII is expressed as an inclusion body and purified by Ni2+-charged resin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood increased levels of activated coagulation factor XIIa are associated with coronary heart disease, sepsis, and diabetes Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor VII + H2O
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 Homo sapiens factor VIIa
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 ?
additional information increased levels of activated coagulation factor XIIa are associated with coronary heart disease, sepsis, and diabetes Homo sapiens ?
-
 ?
prekallikrein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
FXII
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 Homo sapiens