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Literature summary for 3.4.21.35 extracted from

  • Filippou, P.S.; Ren, A.H.; Bala, S.; Papaioannou, M.D.; Brinc, D.; Prassas, I.; Karakosta, T.; Diamandis, E.P.
    Biochemical characterization of human tissue kallikrein 15 and examination of its potential role in cancer (2018), Clin. Biochem., 58, 108-115 .
    View publication on PubMed
Search for more literature for 3.4.21.35

Activating Compound

Activating Compound Comment Organism Structure
serpin A5 about 120% activity at 0.5-1.0 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Expi293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by serpin A3 Homo sapiens
phenylmethylsulfonyl fluoride about 50% residual activity at 2.5 mM Homo sapiens
serpin A1 about 40% residual activity at 0.002 mM Homo sapiens
serpin F2 about 50% residual activity at 0.002 mM Homo sapiens
Zn2+ about 20% residual activity at 0.5 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ enhances the enzyme activity at concentrations of 2.5 and 5 mM Homo sapiens
additional information Mg2+ has no significant effect on the enzyme activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H2R5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has two glycosylated sites within the active protease domain Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
PC-3 cell
-
 Homo sapiens
-
prostate gland cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleaves preferentially after arginine Homo sapiens ?
-
 ?
QAR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens QAR + 7-amino-4-methylcoumarin
-
 ?
VPR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens VPR + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, deglycosylated protein, SDS-PAGE Homo sapiens
? x * 40000, glycosylated protein, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
KLK15
-
 Homo sapiens
tissue kallikrein 15
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 35% activity at pH 7.0, about 90% activity at pH 8.0, about 55% activity at pH 9.0, about 35% activity at pH 10.0 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 at pH 8.0 and 37°C Homo sapiens serpin A1