Cloned (Comment) | Organism |
---|---|
DNA sequence determination, expression of prokallikrein in Pichia pastoris | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | naturally occuring urinary enzyme, pH 8.0, 22°C | Homo sapiens | |
0.099 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | recombinant enzyme from liver, pH 8.0, 22°C | Homo sapiens | |
0.112 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | recombinant enzyme from pancreas, pH 8.0, 22°C | Homo sapiens | |
0.114 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | recombinant enzyme from salivary gland, pH 8.0, 22°C | Homo sapiens | |
0.12 | - |
DL-Val-Leu-Arg-p-nitroanilide | recombinant enzyme from liver, pH 8.0, 22°C | Homo sapiens | |
0.14 | - |
DL-Val-Leu-Arg-p-nitroanilide | natural urinary enzyme, pH 8.0, 22°C | Homo sapiens | |
0.154 | - |
DL-Val-Leu-Arg-p-nitroanilide | recombinant enzyme from salivary gland, pH 8.0, 22°C | Homo sapiens | |
0.158 | - |
DL-Val-Leu-Arg-p-nitroanilide | recombinant enzyme from pancreas, pH 8.0, 22°C | Homo sapiens | |
0.516 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | naturally occuring urinary enzyme, pH 8.0, 22°C | Homo sapiens | |
0.668 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | recombinant enzyme from liver, pH 8.0, 22°C | Homo sapiens | |
0.764 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | recombinant enzyme from salivary gland, pH 8.0, 22°C | Homo sapiens | |
0.842 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | recombinant enzyme from pancreas, pH 8.0, 22°C | Homo sapiens | |
58.8 | - |
DL-Val-Leu-Arg-p-nitroanilide | natural urinary enzyme, pH 8.0, 22°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms | Homo sapiens |
37000 | - |
x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is implicated in the pathology of various inflammatory disorders, the enzyme is an attractive target for therapeutic intervention in diseases such as asthma, pancreatitis and rheumatoid arthritis | ? | - |
? | |
polypeptide + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant from Pichia pastoris | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
pancreas | - |
Homo sapiens | - |
salivary gland | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.53 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-Val-Leu-Arg-p-nitroanilide + H2O | - |
Homo sapiens | DL-Val-Leu-Arg + p-nitroaniline | - |
? | |
low-molecular-weight kininogen + H2O | - |
Homo sapiens | kallidin + kinin | - |
? | |
additional information | the enzyme is implicated in the pathology of various inflammatory disorders, the enzyme is an attractive target for therapeutic intervention in diseases such as asthma, pancreatitis and rheumatoid arthritis | Homo sapiens | ? | - |
? | |
polypeptide + H2O | - |
Homo sapiens | peptides | - |
? | |
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O | - |
Homo sapiens | ? | - |
? | |
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Val-Pro-Phe-thiobenzyl ester + H2O | - |
Homo sapiens | succinyl-Val-Pro-Phe + phenylmethanethiol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
DL-Val-Leu-Arg-p-nitroanilide | recombinant enzyme from liver, pH 8.0, 22°C | Homo sapiens | |
1.07 | - |
DL-Val-Leu-Arg-p-nitroanilide | recombinant salivary enzyme, pH 8.0, 22°C | Homo sapiens | |
1.16 | - |
DL-Val-Leu-Arg-p-nitroanilide | recombinant pancreatic enzyme, pH 8.0, 22°C | Homo sapiens | |
2 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | recombinant enzyme from liver and naturally occuring urinary enzyme, pH 8.0, 22°C | Homo sapiens | |
5.3 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | recombinant enzyme from liver, pH 8.0, 22°C | Homo sapiens | |
6.1 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | naturally occuring urinary enzyme, pH 8.0, 22°C | Homo sapiens | |
7.3 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | recombinant salivary enzyme, pH 8.0, 22°C | Homo sapiens | |
7.4 | - |
Pro-Phe-Arg-4-methylcoumarin 7-amide | recombinant pancreatic enzyme, pH 8.0, 22°C | Homo sapiens | |
27 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | recombinant pancreatic enzyme, pH 8.0, 22°C | Homo sapiens | |
29 | - |
succinyl-Val-Pro-Phe-thiobenzyl ester | recombinant salivary enzyme, pH 8.0, 22°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |