Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.35 extracted from

  • Chan, H.; Springman, E.B.; Clark, J.M.
    Expression and characterization of human tissue kallikrein variants (1998), Protein Expr. Purif., 12, 361-370.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination, expression of prokallikrein in Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.084
-
Pro-Phe-Arg-4-methylcoumarin 7-amide naturally occuring urinary enzyme, pH 8.0, 22°C Homo sapiens
0.099
-
Pro-Phe-Arg-4-methylcoumarin 7-amide recombinant enzyme from liver, pH 8.0, 22°C Homo sapiens
0.112
-
Pro-Phe-Arg-4-methylcoumarin 7-amide recombinant enzyme from pancreas, pH 8.0, 22°C Homo sapiens
0.114
-
Pro-Phe-Arg-4-methylcoumarin 7-amide recombinant enzyme from salivary gland, pH 8.0, 22°C Homo sapiens
0.12
-
DL-Val-Leu-Arg-p-nitroanilide recombinant enzyme from liver, pH 8.0, 22°C Homo sapiens
0.14
-
DL-Val-Leu-Arg-p-nitroanilide natural urinary enzyme, pH 8.0, 22°C Homo sapiens
0.154
-
DL-Val-Leu-Arg-p-nitroanilide recombinant enzyme from salivary gland, pH 8.0, 22°C Homo sapiens
0.158
-
DL-Val-Leu-Arg-p-nitroanilide recombinant enzyme from pancreas, pH 8.0, 22°C Homo sapiens
0.516
-
succinyl-Val-Pro-Phe-thiobenzyl ester naturally occuring urinary enzyme, pH 8.0, 22°C Homo sapiens
0.668
-
succinyl-Val-Pro-Phe-thiobenzyl ester recombinant enzyme from liver, pH 8.0, 22°C Homo sapiens
0.764
-
succinyl-Val-Pro-Phe-thiobenzyl ester recombinant enzyme from salivary gland, pH 8.0, 22°C Homo sapiens
0.842
-
succinyl-Val-Pro-Phe-thiobenzyl ester recombinant enzyme from pancreas, pH 8.0, 22°C Homo sapiens
58.8
-
DL-Val-Leu-Arg-p-nitroanilide natural urinary enzyme, pH 8.0, 22°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms Homo sapiens
37000
-
x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is implicated in the pathology of various inflammatory disorders, the enzyme is an attractive target for therapeutic intervention in diseases such as asthma, pancreatitis and rheumatoid arthritis ?
-
?
polypeptide + H2O Homo sapiens
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from Pichia pastoris Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
pancreas
-
Homo sapiens
-
salivary gland
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.53
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-Val-Leu-Arg-p-nitroanilide + H2O
-
Homo sapiens DL-Val-Leu-Arg + p-nitroaniline
-
?
low-molecular-weight kininogen + H2O
-
Homo sapiens kallidin + kinin
-
?
additional information the enzyme is implicated in the pathology of various inflammatory disorders, the enzyme is an attractive target for therapeutic intervention in diseases such as asthma, pancreatitis and rheumatoid arthritis Homo sapiens ?
-
?
polypeptide + H2O
-
Homo sapiens peptides
-
?
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
Homo sapiens ?
-
?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
?
succinyl-Val-Pro-Phe-thiobenzyl ester + H2O
-
Homo sapiens succinyl-Val-Pro-Phe + phenylmethanethiol
-
?

Subunits

Subunits Comment Organism
? x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
DL-Val-Leu-Arg-p-nitroanilide recombinant enzyme from liver, pH 8.0, 22°C Homo sapiens
1.07
-
DL-Val-Leu-Arg-p-nitroanilide recombinant salivary enzyme, pH 8.0, 22°C Homo sapiens
1.16
-
DL-Val-Leu-Arg-p-nitroanilide recombinant pancreatic enzyme, pH 8.0, 22°C Homo sapiens
2
-
succinyl-Val-Pro-Phe-thiobenzyl ester recombinant enzyme from liver and naturally occuring urinary enzyme, pH 8.0, 22°C Homo sapiens
5.3
-
Pro-Phe-Arg-4-methylcoumarin 7-amide recombinant enzyme from liver, pH 8.0, 22°C Homo sapiens
6.1
-
Pro-Phe-Arg-4-methylcoumarin 7-amide naturally occuring urinary enzyme, pH 8.0, 22°C Homo sapiens
7.3
-
Pro-Phe-Arg-4-methylcoumarin 7-amide recombinant salivary enzyme, pH 8.0, 22°C Homo sapiens
7.4
-
Pro-Phe-Arg-4-methylcoumarin 7-amide recombinant pancreatic enzyme, pH 8.0, 22°C Homo sapiens
27
-
succinyl-Val-Pro-Phe-thiobenzyl ester recombinant pancreatic enzyme, pH 8.0, 22°C Homo sapiens
29
-
succinyl-Val-Pro-Phe-thiobenzyl ester recombinant salivary enzyme, pH 8.0, 22°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens