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Literature summary for 3.4.21.35 extracted from
- Purification of rat urinary kallikrein: comparative studies with rat submandibular gland kallikrein-like serine protease (1992), Prep. Biochem., 22, 239-256.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aprotinin | - |
Rattus norvegicus |  |
| EDTA | - |
Rattus norvegicus | |
| leupeptin | - |
Rattus norvegicus | |
| PMSF | - |
Rattus norvegicus | |
| Soybean trypsin inhibitor | no inhibition | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.103 | - |
tosyl-Gly-Pro-Arg-p-nitroanilide | pH 8.0, 37°C | Rattus norvegicus | |
| 0.45 | - |
benzoyl-Pro-Phe-Arg-p-nitroanilide | pH 8.0, 37°C | Rattus norvegicus | |
| 0.528 | - |
D-Val-Leu-Lys-p-nitroanilide | pH 8.0, 37°C | Rattus norvegicus | |
| 1.83 | - |
DL-Val-Leu-Arg-p-nitroanilide | pH 8.0, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, urinary enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 427fold from submandibular gland | Rattus norvegicus |
| from urine | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submandibular gland | - |
Rattus norvegicus | - |
| urine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | benzoyl-Pro-Phe-Arg + p-nitroaniline | - |
? | |
| D-Val-Leu-Lys-4-nitroanilide + H2O | - |
Rattus norvegicus | D-Val-Leu-Lys + 4-nitroaniline | - |
? | |
| DL-Val-Leu-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | DL-Val-Leu-Arg + p-nitroaniline | - |
? | |
| polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
| tosyl-Gly-Pro-Arg-p-nitroanilide + H2O | - |
Rattus norvegicus | tosyl-Gly-Pro-Arg + p-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, urinary enzyme, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
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