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Literature summary for 3.4.21.34 extracted from

  • Colman, R.W.; Bagdasarian, A.
    Human kallikrein and prekallikrein (1976), Methods Enzymol., 45, 303-322.
    View publication on PubMed
Search for more literature for 3.4.21.34

General Stability

General Stability Organism
IgG stabilizes Homo sapiens
stable to freezing and thawing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin
-
 Homo sapiens
antithrombin III
-
 Homo sapiens
C1 esterase inhibitor
-
 Homo sapiens
diisopropylfluorophosphate
-
 Homo sapiens
diphenylcarbamoylfluoride
-
 Homo sapiens
p-Carboethoxyphenyl epsilon-guanidine caproate
-
 Homo sapiens
Soybean trypsin inhibitor
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00156
-
 Nalpha-toluenesulfonyl-L-arginine methyl ester
-
 Bos taurus
0.0136
-
 Nalpha-benzoyl-L-arginine ethyl ester
-
 Homo sapiens
0.0962
-
 Nalpha-benzoyl-L-arginine ethyl ester
-
 Bos taurus
0.136
-
 Nalpha-toluenesulfonyl-L-arginine methyl ester
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 gel filtration Homo sapiens
95000
-
 gel filtration Bos taurus
99800
-
 sedimentation equilibrium, kallikrein I Homo sapiens
163000
-
 sedimentation equilibrium, kallikrein II Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kininogen + H2O Homo sapiens cleaves Lys-Arg and Arg-Ser bonds in kininogen to produce bradykinin, participates in early phase of intrinsic blood coagulation ?
-
 ?
kininogen + H2O Bos taurus cleaves Lys-Arg and Arg-Ser bonds in kininogen to produce bradykinin, participates in early phase of intrinsic blood coagulation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
 kallikrein I, kallikrein II
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-
plasma
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-50°C, stable for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kininogen + H2O cleaves Lys-Arg and Arg-Ser bonds in kininogen to produce bradykinin, participates in early phase of intrinsic blood coagulation Homo sapiens ?
-
 ?
kininogen + H2O cleaves Lys-Arg and Arg-Ser bonds in kininogen to produce bradykinin, participates in early phase of intrinsic blood coagulation Bos taurus ?
-
 ?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
 Homo sapiens Nalpha-Benzoyl-L-Arg + ethanol
-
 ?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
 Bos taurus Nalpha-Benzoyl-L-Arg + ethanol
-
 ?
Nalpha-toluenesulfonyl-L-arginine methyl ester + H2O
-
 Homo sapiens Nalpha-toluenesulfonyl-L-arginine + methanol
-
 ?
Nalpha-toluenesulfonyl-L-arginine methyl ester + H2O
-
 Bos taurus Nalpha-toluenesulfonyl-L-arginine + methanol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.65
-
-
 Homo sapiens
8.8
-
-
 Bos taurus