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Literature summary for 3.4.21.34 extracted from

  • Gozzo, A.J.; Nunes, V.A.; Cruz-Silva, I.; Carmona, A.K.; Nader, H.B.; Faljoni-Alario, A.; Sampaio, M.U.; Araujo, M.S.
    Heparin modulation of human plasma kallikrein on different substrates and inhibitors (2006), Biol. Chem., 387, 1129-1138.
    View publication on PubMed
Search for more literature for 3.4.21.34

Inhibitors

Inhibitors Comment Organism Structure
alpha1-antiprotease
-
 Homo sapiens
antithrombin inhibition by antithrombin is 3.4fold improved by heparin, forming a ternary complex with antithrombin Homo sapiens
C1-inhibitor inhibition by C1-inhibitor is 1.4fold improved by heparin Homo sapiens
heparin improves inhibition by other enzyme inhibitor 1.4-3.4fold, the enzyme binds to a heparin-matrix, but its secondary structure is not modified by heparin, circular dichroism, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 plasminogen pH 8.0, 37°C Homo sapiens
0.00075
-
 high-molecular-weight kininogen pH 8.0, 37°C Homo sapiens
0.0024
-
 Factor XII pH 8.0, 37°C Homo sapiens
0.0049
-
 2-aminobenzoic acid-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 8.0, 37°C Homo sapiens
0.269
-
 H-D-Pro-Phe-Arg-4-nitroanilide pH 8.0, 37°C Homo sapiens
0.736
-
 acetyl-Phe-Arg-4-nitroanilide pH 8.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 x * 88000 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme involved in intrinsic blood clotting, the kallikrein-kinin system and fibrinolysis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoic acid-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Homo sapiens 2-aminobenzoic acid-Gly-Phe-Ser-Pro-Phe-Arg + Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
acetyl-Phe-Arg-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
factor XII + H2O proteolytic activation Homo sapiens activated factor XII + ?
-
 ?
H-D-Pro-Phe-Arg-4-nitroanilide + H2O i.e. S2302, a chromogenic substrate Homo sapiens ?
-
 ?
high-molecular-weight kininogen + H2O
-
 Homo sapiens high-molecular-weight kinin + bradykinin bradykinin is Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg ?
additional information the enzyme involved in intrinsic blood clotting, the kallikrein-kinin system and fibrinolysis Homo sapiens ?
-
 ?
plasminogen + H2O proteolytic activation Homo sapiens plasmin + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 88000 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00034
-
 plasminogen pH 8.0, 37°C Homo sapiens
0.001
-
 Factor XII pH 8.0, 37°C Homo sapiens
0.0306
-
 high-molecular-weight kininogen pH 8.0, 37°C Homo sapiens
0.1
-
 2-aminobenzoic acid-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 8.0, 37°C Homo sapiens
37.7
-
 acetyl-Phe-Arg-4-nitroanilide pH 8.0, 37°C Homo sapiens
37.7
-
 H-D-Pro-Phe-Arg-4-nitroanilide pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics and secondorder rate constants Homo sapiens