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Literature summary for 3.4.21.34 extracted from

  • Oliva, M.L.; Santomauro-Vaz, E.M.; Andrade, S.A.; Juliano, M.A.; Pott, V.J.; Sampaio, M.U.; Sampaio, C.A.
    Synthetic peptides and fluorogenic substrates related to the reactive site sequence of Kunitz-type inhibitors isolated from Bauhinia: interaction with human plasma kallikrein (2001), Biol. Chem., 382, 109-113.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Bauhinia ungulata factor Xa inhibitor protein
-
Homo sapiens
Bauhinia variegata trypsin inhibitor protein
-
Homo sapiens
o-aminobenzoyl-FESPLRINIIKE-N-(2,4-dinitrophenyl)-ethylene diamine competitive inhibitors, based on the Bauhinia bauhinioides inhibitor protein reactive site sequence Homo sapiens
o-aminobenzoyl-RPGLPVRFESPL-N-(2,4-dinitrophenyl)-ethylene diamine competitive inhibitors, based on the Bauhinia bauhinioides inhibitor protein reactive site sequence Homo sapiens
specific serine protease inhibitor protein from Bauhinia bauhinioides seeds Kunitz type, purified 20 kDa protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
o-aminobenzoyl-VMIAALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine pH 8.0, 37°C Homo sapiens
0.00142
-
o-aminobenzoyl-VVISALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine pH 8.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble plasma Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kininogen + H2O
-
Homo sapiens bradykinin + fragment of kininogen
-
?
additional information no activity with o-aminobenzoyl-RPGLPVRFESPL-N-(2,4-dinitrophenyl)-ethylene diamine and o-aminobenzoyl-FESPLRINIIKE-N-(2,4-dinitrophenyl)-ethylene diamine, which are based on the Bauhinia bauhinioides inhibitor protein reactive site sequence Homo sapiens ?
-
?
o-aminobenzoyl-VMIAALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine + H2O low activity, substrate sequence is based on the Bauhinia ungulata inhibitor protein reactive site sequence Homo sapiens ?
-
?
o-aminobenzoyl-VVISALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine + H2O substrate sequence is based on the Bauhinia variegata inhibitor protein reactive site sequence Homo sapiens ?
-
?
RPGLPVRFESPLRINIIKE + H2O peptide based on the reactive site of the Bauhinia bauhinioides inhibitor protein, similar to the kinin moiety in human kininogen Homo sapiens RPGLPVRFESPLR + INIIKE main products ir
RPGLPVRFESPLRINIIKE + H2O peptide based on the reactive site of the Bauhinia bauhinioides inhibitor protein, similar to the kinin moiety in human kininogen Homo sapiens RPGLPVR + FESPLRINIIKE
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
o-aminobenzoyl-VMIAALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine pH 8.0, 37°C Homo sapiens
3.13
-
o-aminobenzoyl-VVISALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine pH 8.0, 37°C Homo sapiens
3.66
-
o-aminobenzoyl-VVISALPRTMFIQ-N-(2,4-dinitrophenyl)-ethylene diamine pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000035
-
specific serine protease inhibitor protein from Bauhinia bauhinoides seeds pH 8.0, 37°C Homo sapiens
0.0000069
-
Bauhinia ungulata factor Xa inhibitor protein pH 8.0, 37°C Homo sapiens
0.000023
-
Bauhinia variegata trypsin inhibitor protein pH 8.0, 37°C Homo sapiens