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Literature summary for 3.4.21.32 extracted from

  • Li, Q.; Cui, Z.; Liu, Y.; Wang, S.; Song, C.
    Identification and characterization of two novel types of non-clip domain serine proteases (PtSP and PtSPH1) from cDNA haemocytes library of swimming crab Portunus trituberculatus (2012), Fish Shellfish Immunol., 32, 683-692.
    View publication on PubMed
Search for more literature for 3.4.21.32

Cloned(Commentary)

Cloned (Comment) Organism
gene SP, DNA and amino acid sequence determination and analysis, genomic organization, sequence comparison and phylogenetic analysis, quantitative real-time PCR expression analysis Portunus trituberculatus
gene SPH, DNA and amino acid sequence determination and analysis, genomic organization, sequence comparison and phylogenetic analysis, quantitative real-time PCR expression analysis Portunus trituberculatus

Organism

Organism UniProt Comment Textmining
Portunus trituberculatus H8Y6I5 active enzyme variaant PtSP
-
Portunus trituberculatus H8Y6I6 inactive variant PtSPH1
-

Source Tissue

Source Tissue Comment Organism Textmining
eyestalk
-
 Portunus trituberculatus
-
gill
-
 Portunus trituberculatus
-
heart low expression level Portunus trituberculatus
-
hemocyte
-
 Portunus trituberculatus
-
hemocyte very high expression level Portunus trituberculatus
-
additional information the PtSP mRNA is mainly distributed in hemocytes, gill and eyestalk, very low expression in hepatopancreas and stomach, no expression in muscle Portunus trituberculatus
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additional information the PtSPH1 transcript is mainly expressed in stomach, no expression in heart, hepatopancreas and muscle Portunus trituberculatus
-
stomach the PtSPH1 transcript is mainly expressed in stomach Portunus trituberculatus
-

Synonyms

Synonyms Comment Organism
non-clip domain serine protease
-
 Portunus trituberculatus
PtSP
-
 Portunus trituberculatus
PtSPH1
-
 Portunus trituberculatus

Expression

Organism Comment Expression
Portunus trituberculatus the enzyme variant PtSP shows slight increase during the first 48 h compared to control groups except 8 h point after Micrococcus luteus challenge up
Portunus trituberculatus the expression level of enzyme variant PtSPH1 is challenged by Gram-negative bacteria Vibrio alginolyticus, Gram-positive bacteria Micrococcus luteus and fungi Pichia pastoris during the first 48 h up

General Information

General Information Comment Organism
malfunction absence of Ser catalytic residue results in the loss of serine protease activity of PtSPH1 Portunus trituberculatus
additional information the enzyme contains a serine protease domain at the C-terminus Portunus trituberculatus