Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G193A | site-directed mutagenesis, the mutant shows reduced catalytic activity and impaired binding of inhibitors 4-aminobenzamidine and diisopropylfluorophosphate, respectively, indicating distortion of, or altered accessibility to, the S1 and oxyanion-binding sites | Homo sapiens |
G193D | site-directed mutagenesis, the mutant shows reduced catalytic activity and binding of inhibitors 4-aminobenzamidine and diisopropylfluorophosphate impaired 1.6-36fold, respectively, indicating distortion of, or altered accessibility to, the S1 and oxyanion-binding sites | Homo sapiens |
G193E | site-directed mutagenesis, the mutant shows reduced catalytic activity and impaired binding of inhibitors 4-aminobenzamidine and diisopropylfluorophosphate, respectively, indicating distortion of, or altered accessibility to, the S1 and oxyanion-binding sites | Homo sapiens |
G193K | site-directed mutagenesis, the mutant shows reduced catalytic activity and binding of inhibitors 4-aminobenzamidine and diisopropylfluorophosphate impaired 35-478fold, respectively, indicating distortion of, or altered accessibility to, the S1 and oxyanion-binding sites | Homo sapiens |
G193R | site-directed mutagenesis, the mutant shows reduced catalytic activity and impaired binding of inhibitors 4-aminobenzamidine and diisopropylfluorophosphate, respectively, indicating distortion of, or altered accessibility to, the S1 and oxyanion-binding sites | Homo sapiens |
G193V | site-directed mutagenesis, the mutant shows reduced catalytic activity and impaired binding of inhibitors 4-aminobenzamidine and diisopropylfluorophosphate, respectively, indicating distortion of, or altered accessibility to, the S1 and oxyanion-binding sites | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminobenzamidine | beta-branching of the side chain of residue 193 is deleterious for interactions with 4-aminobenzamidine, diisopropylfluorphosphate, and amidolytic substrates, situations where no S2'-P2' interactions are involved, beta-branching causes steric conflicts with the FXIa 140-loop, overview | Homo sapiens | |
amyloid beta-precursor protein Kunitz domain | beta-branching of the side chain of residue 193 is deleterious for interactions with 4-aminobenzamidine, diisopropylfluorphosphate, and amidolytic substrates, situations where no S2'-P2' interactions are involved, beta-branching causes steric conflicts with the FXIa 140-loop, overview | Homo sapiens | |
antithrombin | in presence of heaprin | Homo sapiens | |
diisopropylfluorphosphate | beta-branching of the side chain of residue 193 is deleterious for interactions with 4-aminobenzamidine, diisopropylfluorphosphate, and amidolytic substrates, situations where no S2'-P2' interactions are involved, beta-branching causes steric conflicts with the FXIa 140-loop, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes, overview | Homo sapiens | |
0.26 | - |
pyroGlu-Pro-Arg-4-nitroanilide | recombinant wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
0.34 | - |
D-Ile-Pro-Arg-4-nitroanilide | recombinant wild-type enzyme, pH 7.5, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P03951 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ile-Pro-Arg-4-nitroanilide + H2O | substrate S-2288 | Homo sapiens | D-Ile-Pro-Arg + 4-nitroaniline | - |
? | |
factor IX + H2O | cleavage at Ala145 and Ala180 | Homo sapiens | activated factor IX + ? | - |
? | |
additional information | beta-branching of the side chain of residue 193 is deleterious for interactions with 4-aminobenzamidine, diisopropylfluorphosphate, and amidolytic substrates, situations where no S2'-P2' interactions are involved, overview | Homo sapiens | ? | - |
? | |
pyroGlu-Pro-Arg-4-nitroanilide + H2O | substrate S-2366 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
blood coagulation factor XIa | - |
Homo sapiens |
factor XIa | - |
Homo sapiens |
FXIa | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110.2 | - |
D-Ile-Pro-Arg-4-nitroanilide | recombinant wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
145 | - |
pyroGlu-Pro-Arg-4-nitroanilide | recombinant wild-type enzyme, pH 7.5, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |