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Literature summary for 3.4.21.27 extracted from
- Macromolecular substrate-binding exosites on both the heavy and light chains of factor XIa mediate the formation of the Michaelis complex required for factor IX-activation (2007), Biochemistry, 46, 9830-9839.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the peptide comprising 237 amino acids at the C-terminal end of FXI, peptide FXI/E361-V607, in Pichia pastoris strain X33 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminobenzamidine | a reversible serine protease inhibitor, competitive versus substrate S-2366, noncompetitive versus substrate factor IX, inhibition of wild-type enzyme and recombinant peptide FXI/E361-V607 | Homo sapiens |  |
| S-2366 | noncompetitive inhibition versus factor IX | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | 0.00021 | factor IX | 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.00039 | 0.00047 | factor IX | 37°C, recombinant peptide FXI/E361-V607 | Homo sapiens | |
| 0.225 | - |
S-2366 | 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.357 | - |
S-2366 | 37°C, recombinant peptide FXI/E361-V607 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for proteolytic activity | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor IX + H2O | Homo sapiens | factor XIa is essential for the optimal activation of FIX | activated factor IX + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the zymogen is activated by proteolytic cleavage | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | from plasma | Homo sapiens | - |
| plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor IX + H2O | factor XIa is essential for the optimal activation of FIX | Homo sapiens | activated factor IX + ? | - |
? | |
| factor IX + H2O | cleavage of Arg145-Ala146, and Arg180-Val181, macromolecular substrate-binding exosites on both the heavy and light chains of factor XIa mediate the formation of the Michaelis complex required for factor IX-activation, mechanisms of activation, overview | Homo sapiens | activated factor IX + ? | - |
? | |
| S-2366 + H2O | commercial chromogenic substrate | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | macromolecular substrate-binding exosites on both the heavy and light chains of factor XIa are required for mediation of formation of the Michaelis complex required for factor IX-activation, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| factor XIa | - |
Homo sapiens |
| FXIa | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00065 | - |
factor IX | 37°C, recombinant peptide FXI/E361-V607 | Homo sapiens | |
| 0.334 | - |
factor IX | 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 113 | - |
S-2366 | 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 121 | - |
S-2366 | 37°C, recombinant peptide FXI/E361-V607 | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
4-aminobenzamidine | 37°C, recombinant wild-type enzyme, versus substrate S-2366 | Homo sapiens | |
| 0.085 | - |
4-aminobenzamidine | 37°C, recombinant peptide FXI/E361-V607, versus substrate S-2366 | Homo sapiens | |
| 0.086 | - |
4-aminobenzamidine | 37°C, recombinant peptide FXI/E361-V607, versus substrate factor IX | Homo sapiens | |
| 0.095 | - |
4-aminobenzamidine | 37°C, recombinant wild-type enzyme, versus substrate factor IX | Homo sapiens | |
| 0.42 | - |
S-2366 | 37°C, recombinant peptide FXI/E361-V607, versus substrate factor IX | Homo sapiens | |
| 0.425 | - |
S-2366 | 37°C, recombinant wild-type enzyme, versus substrate factor IX | Homo sapiens | |
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