Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | - |
Coprinopsis clastophylla |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 14% residual activity at 0.2 mM | Coprinopsis clastophylla | |
4-toluenesulfonamide | 98% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Co2+ | 48% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Cu2+ | 13% residual activity at 0.2 mM | Coprinopsis clastophylla | |
diisopropyl fluorophosphate | 13% residual activity at 0.2 mM | Coprinopsis clastophylla | |
EDTA | 93% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Hg2+ | complete inhibition at 0.2 mM | Coprinopsis clastophylla | |
iodoacetamide | 87% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Mn2+ | 93% residual activity at 0.2 mM | Coprinopsis clastophylla | |
N-ethylmaleimide | 50% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Ni2+ | 86% residual activity at 0.2 mM | Coprinopsis clastophylla | |
o-phenanthroline | 79% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Sn2+ | 87% residual activity at 0.2 mM | Coprinopsis clastophylla | |
Zn2+ | 61% residual activity at 0.2 mM | Coprinopsis clastophylla |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.74 | - |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
3.42 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | at pH 8.0 and 37°C | Coprinopsis clastophylla |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates | Coprinopsis clastophylla |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
x * 84000, SDS-PAGE | Coprinopsis clastophylla |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis clastophylla | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, hydroxyapatite column chromatography, and Resource Q column chromatography | Coprinopsis clastophylla |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
supernatant, using benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide as substrate, at pH 8.0 and 37°C | Coprinopsis clastophylla |
52.1 | - |
after 285fold purification, using benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide as substrate, at pH 8.0 and 37°C | Coprinopsis clastophylla |
61.3 | - |
after 285fold purification, using benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin as substrate, at pH 8.0 and 37°C | Coprinopsis clastophylla |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide + H2O | - |
Coprinopsis clastophylla | benzyloxycarbonyl-Gly-L-Pro + 4-nitroaniline | - |
? | |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin + H2O | - |
Coprinopsis clastophylla | benzyloxycarbonyl-Gly-L-Pro + 7-amino-4-methylcoumarin | - |
? | |
additional information | no activity toward L-Pro-4-nitroanilide, Gly-L-Pro-4-nitroanilide, Z-Pro-nitrophenyl ester, Z-Gly-Gly-nitrophenyl ester, Z-Phe-Arg-7-amido-4-methylcoumarin, Z-Leu-Leu-Glu-7-amido-4-methylcoumarin, Z-Val-Lys-Met-7-amido-4-methylcoumarin, Z-Leu-Leu-Leu-7-amido-4-methylcoumarin, and Z-Tyr-Val-Ala-Asp-4-nitroanilide | Coprinopsis clastophylla | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, SDS-PAGE | Coprinopsis clastophylla |
Synonyms | Comment | Organism |
---|---|---|
post-proline cleaving endopeptidase | - |
Coprinopsis clastophylla |
proline-specific endopeptidase | - |
Coprinopsis clastophylla |
prolyl endopeptidase | - |
Coprinopsis clastophylla |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Coprinopsis clastophylla |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | the enzyme is active over a temperature range of 25-37°C | Coprinopsis clastophylla |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the enzyme retains about 20% of its activity after incubation at 45°C for 30 min | Coprinopsis clastophylla |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.6 | - |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
162 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | at pH 8.0 and 37°C | Coprinopsis clastophylla |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Coprinopsis clastophylla |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the activity of the enzyme is reduced by 50% and 60% at pH 6.0 and 9.0, respectively, compared to its maximal activity | Coprinopsis clastophylla |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Coprinopsis clastophylla | isoelectric focusing | - |
5.2 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.2 | - |
at pH 8.0 and 37°C | Coprinopsis clastophylla | N-ethylmaleimide |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.1 | - |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
47.4 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | at pH 8.0 and 37°C | Coprinopsis clastophylla |