Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 34.5% residual activity at 1 mM | Aspergillus oryzae | |
Cu2+ | 14.1% residual activity at 1 mM | Aspergillus oryzae | |
Fe2+ | 80.6% residual activity at 1 mM | Aspergillus oryzae | |
K+ | 92.2% residual activity at 1 mM | Aspergillus oryzae | |
Mg2+ | 87.3% residual activity at 1 mM | Aspergillus oryzae | |
Mn2+ | 24.3% residual activity at 1 mM | Aspergillus oryzae | |
Na+ | 93.1% residual activity at 1 mM | Aspergillus oryzae | |
Zn2+ | 31.2% residual activity at 1 mM | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
L-Ala-L-Ala-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae | |
0.37 | - |
Z-Gly-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae | |
0.56 | - |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ shows activating effect on enzyme activity | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
x * 64000, SDS-PAGE | Aspergillus oryzae |
64800 | - |
x * 64800, calculated from amino acid sequence | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae WX2011 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap DEAE column chromatography | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | L-Ala-L-Ala-L-Pro + 4-nitroaniline | - |
? | |
L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae WX2011 | L-Ala-L-Ala-L-Pro + 4-nitroaniline | - |
? | |
Z-Gly-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | Z-Gly-L-Pro + 4-nitroaniline | - |
? | |
Z-Gly-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae WX2011 | Z-Gly-L-Pro + 4-nitroaniline | - |
? | |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline | - |
? | |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae WX2011 | Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Aspergillus oryzae |
? | x * 64800, calculated from amino acid sequence | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
PEP | - |
Aspergillus oryzae |
prolyl endopeptidase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | the enzyme retains over 80% activity after incubation for 30 min at 40°C. The enzyme is not stable at 65 and 70°C, decreasing to 40 and 32% maximum activity, respectively. The activity is almost lost after incubation of 30 min at 80°C | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93 | - |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae | |
105.7 | - |
Z-Gly-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae | |
139.4 | - |
L-Ala-L-Ala-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | over 60% of the maximum activity is retained between pH 3.0 and 8.0 | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | within the pH range of 3.5-7.0, the enzyme retains more than 80% of the initial activity after incubation at 37°C for 30 min | Aspergillus oryzae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus oryzae | calculated from amino acid sequence | - |
5.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
166.1 | - |
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae | |
285.7 | - |
Z-Gly-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae | |
496.4 | - |
L-Ala-L-Ala-L-Pro-4-nitroanilide | at 40°C and pH 4.0 | Aspergillus oryzae |