Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T202C | the mutation increases the flexibility of loop A, enhancing the catalytic efficiency 2fold beyond that of the native enzyme | Homo sapiens |
T202C/T590C | the mutant shows more than 10fold increased Km and increased kcat values towards Abz-Gly-L-Phe-L-Arg-L-Pro-L-Phe(NO2)-L-Arg-L-Ala at pH 8.0 compared to the wild type enzyme | Homo sapiens |
T590C | variant displays an altered substrate preference profile, with reduced ligand binding capacity. The mutation in loop B increased the preference for shorter peptides, indicating a role in substrate gating | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KYP-2047 | i.e. 4-phenylbutanoyl-l-prolyl-2(S)-cyanopyrrolidine, highly potent covalent reversible competitive inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00081 | - |
Abz-Gly-L-Phe-L-Arg-L-Pro-L-Phe(NO2)-L-Arg-L-Ala | wild type enzyme, at pH 8.0 and 25°C | Homo sapiens | |
0.00921 | - |
Abz-Gly-L-Phe-L-Arg-L-Pro-L-Phe(NO2)-L-Arg-L-Ala | mutant enzyme T202C/T590C, at pH 8.0 and 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Glu-Gly-L-Phe-Gly-L-Pro-L-Phe-Gly-L-4-nitrophenylalanine-L-Ala + H2O | - |
Homo sapiens | 2-aminobenzoyl-Glu-Gly-L-Phe-Gly-L-Pro + L-Phe-Gly-L-4-nitrophenylalanine-L-Ala | - |
? | |
2-aminobenzoyl-Gly-L-Phe-Gly-L-Pro-L-Phe-Gly-L-4-nitrophenylalanine-L-Ala + H2O | - |
Homo sapiens | 2-aminobenzoyl-Gly-L-Phe-Gly-L-Pro + L-Phe-Gly-L-4-nitrophenylalanine-L-Ala | - |
? | |
2-aminobenzoyl-Gly-L-Phe-L-Arg-L-Pro-L-4-nitrophenylalanine-L-Arg-L-Ala + H2O | - |
Homo sapiens | 2-aminobenzoyl-Gly-L-Phe-L-Arg-L-Pro + L-4-nitrophenylalanine-L-Arg-L-Ala | - |
? | |
2-aminobenzoyl-L-Ser-L-Pro-L-4-nitrophenylalanine-L-Ala + H2O | - |
Homo sapiens | 2-aminobenzoyl-L-Ser-L-Pro + 4-nitrophenylalanine-L-Ala | - |
? | |
Abz-Gly-L-Phe-L-Arg-L-Pro-L-Phe(NO2)-L-Arg-L-Ala + H2O | - |
Homo sapiens | Abz-Gly-L-Phe-L-Arg-L-Pro + L-Phe(NO2)-L-Arg-L-Ala | - |
? | |
Abz-Gly-L-Phe-L-Ser-L-Pro-L-Phe-L-Arg-L-Ser-L-Ser-L-Arg-L-Ile-Gly-L-Glu-L-Ile-L-Lys-L-Glu-L-Glu-L-Gln-N-(2,4-dinitrophenyl)-ethylenediamine + H2O | - |
Homo sapiens | Abz-Gly-L-Phe-L-Ser-L-Pro + L-Phe-L-Arg-L-Ser-L-Ser-L-Arg-L-Ile-Gly-L-Glu-L-Ile-L-Lys-L-Glu-L-Glu-L-Gln-N-(2,4-dinitrophenyl)-ethylenediamine | - |
? | |
Z-Gly-Pro-2-naphthylamide + H2O | - |
Homo sapiens | Z-Gly-Pro + 2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PEP | - |
Homo sapiens |
POP | - |
Homo sapiens |
PREP | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
Abz-Gly-L-Phe-L-Arg-L-Pro-L-Phe(NO2)-L-Arg-L-Ala | wild type enzyme, at pH 8.0 and 25°C | Homo sapiens | |
1.31 | - |
Abz-Gly-L-Phe-L-Arg-L-Pro-L-Phe(NO2)-L-Arg-L-Ala | mutant enzyme T202C/T590C, at pH 8.0 and 25°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000015 | - |
wild type enzyme, at pH 8.0 and 25°C | Homo sapiens | KYP-2047 |