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Literature summary for 3.4.21.26 extracted from
- Characterization of a prolyl endoprotease from Eurygaster integriceps Puton (Sunn pest) infested wheat (2010), Arch. Insect Biochem. Physiol., 74, 163-178.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylmethylsulfonyl fluoride | - |
Eurygaster integriceps |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06528 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | in 25 mM ethanolamine, pH 8.5, at 20°C | Eurygaster integriceps | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
x * 97000, SDS-PAGE | Eurygaster integriceps |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gluten + H2O | Eurygaster integriceps | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eurygaster integriceps | - |
sunn pest | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, Cu2+ Sepharose column chromatography, and Sephacryl S-100 gel filtration | Eurygaster integriceps |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
crude extract and purified enzyme with gluten as substrate show specific activities of 1.0 and 4.5 units/mg protein, respectively, at pH 8.5 and 20°C (one unit is defined as change in gel height of 1 mm/h) | Eurygaster integriceps |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide + H2O | - |
Eurygaster integriceps | benzyloxycarbonyl-Gly-L-Pro + 4-nitroaniline | - |
? | |
| gluten + H2O | - |
Eurygaster integriceps | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 97000, SDS-PAGE | Eurygaster integriceps |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutenase | - |
Eurygaster integriceps |
| prolyl endoprotease | - |
Eurygaster integriceps |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 34 | - |
Eurygaster integriceps |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | 50 | approximately, 50% of the enzyme activity is retained at 38°C during the 20 min period of the assay. The enzyme also retains about 30% of its activity at 40°C but loses its activity entirely at 50°C | Eurygaster integriceps |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | between 20°C and 35°C, above pH 10, the activity declines sharply | Eurygaster integriceps |
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Release 2023.1 (January 2023)
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