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Literature summary for 3.4.21.26 extracted from

  • Ehren, J.; Govindarajan, S.; Moron, B.; Minshull, J.; Khosla, C.
    Protein engineering of improved prolyl endopeptidases for celiac sprue therapy (2008), Protein Eng. Des. Sel., 21, 699-707.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.26

Application

Application Comment Organism
additional information oral therapeutic candidate for protecting celiac sprue patients from the toxic effects of dietary gluten Novosphingobium capsulatum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Novosphingobium capsulatum

Protein Variants

Protein Variants Comment Organism
F227Y mutant of SC PEP Novosphingobium capsulatum
F459Y mutant of SC PEP Novosphingobium capsulatum
I371V mutant of SC PEP Novosphingobium capsulatum
I406V one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
I514V one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
I581V mutant of SC PEP Novosphingobium capsulatum
I687V mutant of SC PEP Novosphingobium capsulatum
K103T one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
M511L one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
additional information mutants of SC PEP, variants with as much as 20% enhanced specific activity at pH 4.5 and 200fold greater resistance to pepsin are identified Novosphingobium capsulatum
R100K mutant of SC PEP Novosphingobium capsulatum
R354K mutant of SC PEP Novosphingobium capsulatum
R481K mutant of SC PEP Novosphingobium capsulatum
S351P mutant of SC PEP Novosphingobium capsulatum
V323I mutant of SC PEP Novosphingobium capsulatum
V346I mutant of SC PEP Novosphingobium capsulatum
V474I one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions Novosphingobium capsulatum
V500I mutant of SC PEP Novosphingobium capsulatum
Y669F mutant of SC PEP Novosphingobium capsulatum

General Stability

General Stability Organism
Enhancing the activity and stability of PEPs under gastric conditions (low pH, high pepsin concentration) is a challenge for protein engineers Novosphingobium capsulatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Novosphingobium capsulatum ability to cleave immunotoxic gluten peptides endoproteolytically, attractive oral therapeutic candidates for protecting celiac sprue patients from the toxic effects of dietary gluten ?
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 ?

Organism

Organism UniProt Comment Textmining
Novosphingobium capsulatum Q9ZNM8
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Novosphingobium capsulatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ability to cleave immunotoxic gluten peptides endoproteolytically, attractive oral therapeutic candidates for protecting celiac sprue patients from the toxic effects of dietary gluten Novosphingobium capsulatum ?
-
 ?
succinyl-Ala-Pro-p-nitroanilide + H2O
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 Novosphingobium capsulatum ?
-
 ?

Synonyms

Synonyms Comment Organism
prolyl endopeptidase
-
 Novosphingobium capsulatum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 variants with as much as 20% enhanced specific activity at pH 4.5 and 200fold greater resistance to pepsin identified Novosphingobium capsulatum