Crystallization (Comment) | Organism |
---|---|
D641A and D641N mutants are cocrystallized in presence of substrate benzyloxycarbonyl-Gly-Pro-4-nitroanilide | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
D641A | the ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-beta-naphthylamide is 0.2% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 is 1.8fold higher than the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-p-nitrophenol is 66% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-SBzl is 0.92% of the wild-type ratio | Sus scrofa |
D641N | the ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-beta-naphthylamide is 0.02% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 is 1.4fold higher than the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-p-nitrophenol is 40% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-SBzl is 0.27% of the wild-type ratio | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-Gly-Pro-CH2Cl | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | pH 7.0, mutant enzyme D641A | Sus scrofa | |
0.000028 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | pH 7.0, mutant enzyme D641N | Sus scrofa | |
0.00152 | - |
benzyloxycarbonyl-Gly-Pro-SBzl | pH 7.0, mutant enzyme D641N | Sus scrofa | |
0.00159 | - |
benzyloxycarbonyl-Gly-Pro-SBzl | pH 7.0, mutant enzymeD641A | Sus scrofa | |
0.00205 | - |
2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 | pH 8.0, wild-type enzyme | Sus scrofa | |
0.0054 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | pH 7.0, mutant enzyme D641A | Sus scrofa | |
0.0059 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | pH 7.0, wild-type enzyme | Sus scrofa | |
0.0135 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | pH 7.0, mutant enzyme D641N | Sus scrofa | |
0.016 | - |
benzyloxycarbonyl-Gly-Pro-SBzl | pH 7.0, wild-type enzyme | Sus scrofa | |
0.0192 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenyl ester | pH 7.0, wild-type enzyme | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P23687 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 + H2O | - |
Sus scrofa | 2-aminobenzoyl-Gly-Phe-Gly-Pro + Phe-Gly-Phe(NO2)-Ala-NH2 | - |
? | |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide + H2O | - |
Sus scrofa | benzyloxycarbonyl-Gly-Pro + 2-naphthylamine | - |
? | |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol + H2O | - |
Sus scrofa | benzyloxycarbonyl-Gly-Pro + p-nitrophenol | - |
? | |
benzyloxycarbonyl-Gly-Pro-SBzl + H2O | - |
Sus scrofa | benzyloxycarbonyl-Gly-Pro + phenyl-methanethiol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0151 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | pH 7.0, mutant enzyme D641N | Sus scrofa | |
0.021 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | pH 7.0, mutant enzyme D641N | Sus scrofa | |
0.028 | - |
benzyloxycarbonyl-Gly-Pro-SBzl | pH 7.0, mutante enzyme D641N | Sus scrofa | |
0.031 | - |
benzyloxycarbonyl-Gly-Pro-p-nitrophenol | pH 7.0, mutant enzyme D641A | Sus scrofa | |
0.0657 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | pH 7.0, mutant enzyme D641A | Sus scrofa | |
0.099 | - |
benzyloxycarbonyl-Gly-Pro-SBzl | pH 7.0, mutant enzymeD641A | Sus scrofa | |
3 | 6 | benzyloxycarbonyl-Gly-Pro-p-nitrophenol | pH 7.0, wild-type enzyme | Sus scrofa | |
3.3 | - |
2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 | pH 8.0, wild-type enzyme | Sus scrofa | |
5.38 | - |
2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 | pH 8.0, wild-type enzyme | Sus scrofa | |
32.5 | - |
benzyloxycarbonyl-Gly-Pro-beta-naphthylamide | pH 7.0, wild-type enzyme | Sus scrofa | |
108 | - |
benzyloxycarbonyl-Gly-Pro-SBzl | pH 7.0, wild-type enzyme | Sus scrofa |