General Stability | Organism |
---|---|
the stability of the 67000 Da and the 54000 Da enzyme is almost the same | Cucumis melo |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha2-Macroglobulin | 38% inhibition by a concentration of 0.001 mM with casein as substrate, and 18% inhibition with succinyl-Ala-Pro-Ala-p-nitroanilide as substrate | Cucumis melo | |
diisopropyl fluorophosphate | complete inhibition by 1 mM | Cucumis melo | |
additional information | no effect of leupeptin, alpha1-antitrypsin, soybean trypsin inhibitor, ovomucoid and aprotinin | Cucumis melo |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | - |
Cucumis melo | |
1.25 | - |
glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide | - |
Cucumis melo | |
1.96 | - |
succinyl-Ala-Pro-Ala-p-nitroanilide | - |
Cucumis melo | |
2.72 | - |
succinyl-Ala-Ala-Ala-p-nitroanilide | - |
Cucumis melo | |
2.91 | - |
succinyl-Ala-Ala-p-nitroanilide | - |
Cucumis melo | |
3.57 | - |
succinyl-Ala-p-nitroanilide | - |
Cucumis melo |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
SDS-PAGE | Cucumis melo |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Protein + H2O | Cucumis melo | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis melo | - |
L. var Prince | - |
Purification (Comment) | Organism |
---|---|
a quick purification method leads to an enzyme fraction with almost only the 67000 Da enzyme | Cucumis melo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-mating factor + H2O | cleaves at one site, Glu-Leu | Cucumis melo | ? | - |
? | |
angiotensin I + H2O | cleaves at two sites, Tyr-Ile and Phe-His | Cucumis melo | ? | - |
? | |
casein + H2O | - |
Cucumis melo | ? | - |
? | |
Cytochrome c + H2O | initial cleavage site, Glu-Lys and Ala-Ala | Cucumis melo | ? | - |
? | |
delta sleep-inducing peptide + H2O | cleaves at one site, Ala-Ser | Cucumis melo | ? | - |
? | |
glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O | - |
Cucumis melo | ? | - |
? | |
insulin + H2O | initial cleavage site, Gln-His | Cucumis melo | ? | - |
? | |
Insulin B-chain + H2O | three cleavage sites are detected after a reaction period of 30 s, Leu-Cys, Cys-Gly, and Glu-Ala | Cucumis melo | ? | - |
? | |
additional information | the substrate specificity of the 67000 Da and the 54000 Da enzyme is almost the same | Cucumis melo | ? | - |
? | |
Myoglobin + H2O | initial cleavage site, Asp-Ile and Glu-Ala | Cucumis melo | ? | - |
? | |
ovalbumin + H2O | initial cleavage site, Asn-Ala | Cucumis melo | ? | - |
? | |
Protein + H2O | - |
Cucumis melo | ? | - |
? | |
protein + H2O | - |
Cucumis melo | hydrolyzed protein | - |
? | |
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O | - |
Cucumis melo | ? | - |
? | |
succinyl-Ala-Ala-p-nitroanilide + H2O | - |
Cucumis melo | ? | - |
? | |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | - |
Cucumis melo | ? | - |
? | |
succinyl-Ala-p-nitroanilide + H2O | - |
Cucumis melo | succinyl-Ala + 4-nitroaniline | - |
? | |
succinyl-Ala-Pro-Ala-p-nitroanilide + H2O | - |
Cucumis melo | ? | - |
? | |
superoxide dismutase + H2O | initial cleavage site, His-Gly and Leu-Ala | Cucumis melo | ? | - |
? | |
TMA-lysozyme BrCN fragment + H2O | cleaves at two sites, Cys-Lys and Arg-Gly | Cucumis melo | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | fairly stable at pH 7.1 up to | Cucumis melo |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
succinyl-Ala-p-nitroanilide | - |
Cucumis melo | |
0.007 | - |
succinyl-Ala-Ala-p-nitroanilide | - |
Cucumis melo | |
0.013 | - |
succinyl-Ala-Ala-Ala-p-nitroanilide | - |
Cucumis melo | |
0.31 | - |
succinyl-Ala-Pro-Ala-p-nitroanilide | - |
Cucumis melo | |
4.17 | - |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | - |
Cucumis melo | |
5.9 | - |
glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide | - |
Cucumis melo |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
11 | - |
retains about 80% of the activity | Cucumis melo |