Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.22 extracted from

  • Hartmann, R.; Dockal, M.; Kammlander, W.; Panholzer, E.; Nicolaes, G.A.; Fiedler, C.; Rosing, J.; Scheiflinger, F.
    Factor IX mutants with enhanced catalytic activity (2009), J. Thromb. Haemost., 7, 1656-1662.
    View publication on PubMed
Search for more literature for 3.4.21.22

Activating Compound

Activating Compound Comment Organism Structure
FVIII in absence of FVIIIa decreased catalytic activity of enzyme FIXa Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y94F/A95aK/K98T/Y177F mutation, higher clotting activity than wild type FIXa Homo sapiens
Y94F/A95aK/K98T/Y177F/I213V/E219G about 6fold increased catalytic efficence compared to plasma-derived activated coagulation factor IX, higher clotting activity than wild type FIXa Homo sapiens
Y94F/K98T increased catalytic efficence compared to plasma-derived activated coagulation factor IX, higher clotting activity than wild type FIXa Homo sapiens
Y94F/K98T/Y177F increased catalytic efficence compared to plasma-derived activated coagulation factor IX, higher clotting activity than wild type FIXa Homo sapiens
Y94F/K98T/Y177F/I213V/E219G about 17fold increased catalytic efficence compared to plasma-derived activated coagulation factor IX, higher clotting activity than wild type FIXa Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000011
-
 Factor X wild type FIXa, FX activation in presence of FVIIIa Homo sapiens
0.000013
-
 Factor X mutant Y94F/A95aK/K98T/Y177F/I213V/E219G, FX activation in presence of FVIIIa Homo sapiens
0.000013
-
 Factor X plasma-derived FIXa, FX activation in presence of FVIIIa Homo sapiens
0.000014
-
 Factor X mutant Y94F/K98T/Y177F, FX activation in presence of FVIIIa Homo sapiens
0.000015
-
 Factor X mutant Y94F/K98T, FX activation in presence of FVIIIa Homo sapiens
0.000017
-
 Factor X mutant Y94F/K98T/Y177F/I213V/E219, FX activation in presence of FVIIIa Homo sapiens
0.00004
-
 Factor X mutant Y94F/K98T/Y177F/I213V/E219, FX activation in absence of FVIIIa Homo sapiens
0.000055
-
 Factor X mutant Y94F/A95aK/K98T/Y177F/I213V/E219G, FX activation in absence of FVIIIa Homo sapiens
0.000065
-
 Factor X plasma-derived FIXa, FX activation in absence of FVIIIa Homo sapiens
0.000068
-
 Factor X mutant Y94F/K98T/Y177F, FX activation in absence of FVIIIa Homo sapiens
0.000068
-
 Factor X wild type FIXa, FX activation in absence of FVIIIa Homo sapiens
0.000083
-
 Factor X mutant Y94F/K98T, FX activation in absence of FVIIIa Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 value about, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Leu-Phg-Arg-4-nitroanilide + H2O Homo sapiens
-
 D-Leu-Phg-Arg + 4-nitroaniline
-
 ?
factor X Homo sapiens activation of factor X to factor Xa ?
-
 ?
methylsulfonyl-D-cyclohexylglycyl-Arg-7-amido-4-methylcoumarin + H2O Homo sapiens assay at 37°C methylsulfonyl-D-cyclohexylglycyl-Arg + 7-amino-4-methylcoumarin
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
combination of anion exchange and affinity/cation exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Leu-Phg-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Leu-Phg-Arg + 4-nitroaniline
-
 ?
factor X activation of factor X to factor Xa Homo sapiens ?
-
 ?
methylsulfonyl-D-cyclohexylglycyl-Arg-7-amido-4-methylcoumarin + H2O assay at 37°C Homo sapiens methylsulfonyl-D-cyclohexylglycyl-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
activated coagulation factor IX
-
 Homo sapiens
FIXa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00011
-
 Factor X mutant Y94F/K98T/Y177F, FX activation in presence of FVIIIa Homo sapiens
0.00017
-
 Factor X wild type FIXa, FX activation in presence of FVIIIa Homo sapiens
0.0002
-
 Factor X mutant Y94F/A95aK/K98T/Y177F/I213V/E219G, FX activation in presence of FVIIIa Homo sapiens
0.00021
-
 Factor X plasma-derived FIXa, FX activation in absence of FVIIIa Homo sapiens
0.00022
-
 Factor X plasma-derived FIXa, FX activation in presence of FVIIIa Homo sapiens
0.00029
-
 Factor X wild type FIXa, FX activation in absence of FVIIIa Homo sapiens
0.0003
-
 Factor X mutant Y94F/K98T, FX activation in presence of FVIIIa Homo sapiens
0.00033
-
 Factor X mutant Y94F/K98T/Y177F, FX activation in absence of FVIIIa Homo sapiens
0.00059
-
 Factor X mutant Y94F/K98T, FX activation in absence of FVIIIa Homo sapiens
0.00088
-
 Factor X mutant Y94F/K98T/Y177F/I213V/E219, FX activation in presence of FVIIIa Homo sapiens
0.001
-
 Factor X mutant Y94F/A95aK/K98T/Y177F/I213V/E219G, FX activation in absence of FVIIIa Homo sapiens
0.0022
-
 Factor X mutant Y94F/K98T/Y177F/I213V/E219, FX activation in absence of FVIIIa Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00011
-
 D-Leu-Phg-Arg-4-nitroanilide mutant Y94F/K98T/Y177F Homo sapiens
0.00011
-
 D-Leu-Phg-Arg-4-nitroanilide plasma-derived FIXa Homo sapiens
0.00012
-
 D-Leu-Phg-Arg-4-nitroanilide mutant Y94F/A95aK/K98T/Y177F/I213V/E219G Homo sapiens
0.00015
-
 D-Leu-Phg-Arg-4-nitroanilide wild type FIXa Homo sapiens
0.00025
-
 D-Leu-Phg-Arg-4-nitroanilide mutant Y94F/K98T/Y177F/I213V/E219 Homo sapiens
0.00037
-
 D-Leu-Phg-Arg-4-nitroanilide mutant Y94F/K98T Homo sapiens
0.83
-
 Factor X mutant Y94F/K98T/Y177F, FX activation in presence of FVIIIa Homo sapiens
1.5
-
 Factor X mutant Y94F/A95aK/K98T/Y177F/I213V/E219G, FX activation in presence of FVIIIa Homo sapiens
1.5
-
 Factor X wild type FIXa, FX activation in presence of FVIIIa Homo sapiens
1.67
-
 Factor X plasma-derived FIXa, FX activation in presence of FVIIIa Homo sapiens
2
-
 Factor X mutant Y94F/K98T, FX activation in presence of FVIIIa Homo sapiens
3.17
-
 Factor X plasma-derived FIXa, FX activation in absence of FVIIIa Homo sapiens
4.17
-
 Factor X wild type FIXa, FX activation in absence of FVIIIa Homo sapiens
4.83
-
 Factor X mutant Y94F/K98T/Y177F, FX activation in absence of FVIIIa Homo sapiens
5.33
-
 Factor X mutant Y94F/K98T/Y177F/I213V/E219, FX activation in presence of FVIIIa Homo sapiens
7.17
-
 Factor X mutant Y94F/K98T, FX activation in absence of FVIIIa Homo sapiens
18.17
-
 Factor X mutant Y94F/A95aK/K98T/Y177F/I213V/E219G, FX activation in absence of FVIIIa Homo sapiens
55
-
 Factor X mutant Y94F/K98T/Y177F/I213V/E219, FX activation in absence of FVIIIa Homo sapiens