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Literature summary for 3.4.21.22 extracted from
- Contribution of basic residues of the autolysis loop to the substrate and inhibitor specificity of factor IXa (2003), J. Biol. Chem., 278, 25032-25038.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Factor VIIIa | - |
Homo sapiens | |
| phospholipid membrane | - |
Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in HEK293 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Gla-domainless factor IXa | 20000times lower kacat for factor X hydrolysis than wild-type | Homo sapiens |
| R143A | 4times lower kcat for factor X hydrolysis than wild-type Gla-domainless factor IXa | Homo sapiens |
| R147A | 3times lower kcat for factor X hydrolysis than wild-type Gla-domainless factor IXa | Homo sapiens |
| R150 | similar kcat for factor X hydrolysis as wild-type Gla-domainless factor IXa | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
CH3SO2-D-Leu-Gly-L-Arg-p-nitroanilide | - |
Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor X + H2O | Homo sapiens | - |
factor Xa | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00740 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant factor IXa, immunoaffinity chromatography using HPC4 monoclonal antibodies | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CH3SO2-D-Leu-Gly-L-Arg-p-nitroanilide + H2O | - |
Homo sapiens | CH3SO2-D-Leu-Gly-L-Arg + p-nitroaniline | - |
? | |
| factor X + H2O | - |
Homo sapiens | factor Xa | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FIXa | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
Factor X | - |
Homo sapiens | |
| 13.2 | - |
CH3SO2-D-Leu-Gly-L-Arg-p-nitroanilide | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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