Literature summary for 3.4.21.22 extracted from

Blostein, M.D.; Rigby, A.C.; Furie, B.C.; Furie, B.; Gilbert, G.E.
Amphipathic helices support function of blood coagulation factor IXa (2000), Biochemistry, 39, 12000-12006.

Search for more literature for 3.4.21.22

Activating Compound

Activating Compound	Comment	Organism	Structure
Factor VIIIa	200000fold activation in the presence of phospholipid membrane through a combinantion of a decrease in Km and an increase in kcat	Homo sapiens
GIGAVLKVLTTGLPALISSWIKRKRQQ	approx. 600fold lower km, slight increase in kcat	Homo sapiens
phospholipid membrane	200000fold activation in the presence of factor VIIIa through a combinantion of a decrease in Km and an increase in kcat	Homo sapiens
TRYLRIHPQSWVHQIALRMEV	approx. 600fold lower km, slight increase in kcat, optimal activation in the presence of 0.5-1 mM Ca2+	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00013	-	Factor X	25°C, pH 7.4, in the presence of GIGAVLKVLTTGLPALISSWIKRKRQQ	Homo sapiens
0.00017	-	Factor X	25°C, pH 7.4, in the presence of TRYLRIHPQSWVHQIALRMEV	Homo sapiens
0.095	-	Factor X	25°C, pH 7.4	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	5 mM, optimal concentration for cleavage of factor X by factor IXa on phospholipid membranes	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
factor X + H2O	Homo sapiens	-	fragments of factor X	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
factor X + H2O	-	Homo sapiens	fragments of factor X	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00016	-	Factor X	25°C, pH 7.4	Homo sapiens
0.00023	-	Factor X	25°C, pH 7.4, in the presence of GIGAVLKVLTTGLPALISSWIKRKRQQ	Homo sapiens
0.00025	-	Factor X	25°C, pH 7.4, in the presence of TRYLRIHPQSWVHQIALRMEV	Homo sapiens

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Release 2023.1 (January 2023)