Literature summary for 3.4.21.21 extracted from

Masroori, N.; Halabian, R.; Mohammadipour, M.; Roushandeh, A.M.; Rouhbakhsh, M.; Najafabadi, A.J.; Fathabad, M.E.; Salimi, M.; Shokrgozar, M.A.; Roudkenar, M.H.
High-level expression of functional recombinant human coagulation factor VII in insect cells (2010), Biotechnol. Lett., 32, 803-809.

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Application

Application	Comment	Organism
biotechnology	to overcome the defect for high-level expression of the functional recombinant coagulation FVII in Sf9 cell this method involves simultaneous expression of both human c-carboxylase and human FVII genes in the host leading to a high-level expression of functional recombinant factor VII	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Spodoptera frugiperda Sf9 cell line using the baculovirus expression system. Recombinant human FVII (rhFVII) produced in this study carries a 6x-His tag at the C-terminal	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE, recombinant fusion protein	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P08709	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	liver cDNA used for cloning	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
FVII	-	Homo sapiens

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Release 2023.1 (January 2023)