Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in BHK cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M306D | mutation abolishes the allosteric linkage between the active site and the binding interface with tissue factor. 2fold increase in amidolytic activity. In sharp contrast to the wild-type enzyme its binding kinetic to soluble tissue factor are unaltered after inactivation with D-Phe-Phe-Arg chloromethyl ketone | Homo sapiens |
M306N | amidolytic activity of the mutant enzyme is stimulated 7fold | Homo sapiens |
M306N/N309S | amidolytic activity of the mutant enzyme is stimulated 1.5fold | Homo sapiens |
M306S | amidolytic activity of the mutant enzyme is stimulated 9fold | Homo sapiens |
M306T | amidolytic activity of the mutant enzyme is stimulated 12fold | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
Factor X | mutant enzyme M306D | Homo sapiens | |
0.00105 | - |
Factor X | wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | - |
Homo sapiens | activated factor X + ? | - |
? |