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Literature summary for 3.4.21.21 extracted from

  • Toso, R.; Bernardi, F.; Tidd, T.; Pinotti, M.; Camire, R.M.; Marchetti, G.; High, K.A.; Pollak, E.S.
    Factor VII mutant V154G models a zymogen-like form of factor VIIa (2003), Biochem. J., 369, 563-571.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.21

Protein Variants

Protein Variants Comment Organism
D343H mutant enzyme shows no activity with methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide, factor X and factor IX Homo sapiens
R152Q mutant enzyme shows no activity with methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide, factor X and factor IX Homo sapiens
S344A mutant enzyme shows no activity with methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide, factor X and factor IX Homo sapiens
V154A mutant enzyme shows reduced proteolytic activity towards factor X and undetectable activity towards factor IX Homo sapiens
V154G naturally occuring mutation, mutant enzyme with a zymogen-like form, markedly reduced activity towards peptidyl substrate and undetectable activity towards macromolecular substrates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the ratio of turnover number to KM-value for the substrate methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide is 25000 for wild-type enzyme, 500 for mutant enzyme V154G and 2500 for mutant enzyme V154A Homo sapiens
0.67
-
 methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification proteolytic cleavage of the peptide bond between Arg152 and Ile153 converts the procoagulant protein factor VII to an activated two-chain form. The formation of a salt bridge between Ile153 and Asp343 drives the conversion to the active form Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor IX + H2O
-
 Homo sapiens activated factor IX + ?
-
 ?
factor X + H2O
-
 Homo sapiens activated factor X + ?
-
 ?
methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide + H2O
-
 Homo sapiens methanesulfonyl-D-cyclohexylalanyl-butyl-arginine + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
FVII
-
 Homo sapiens
procoagulant protein factor VII
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the ratio of turnover number to KM-value for the substrate methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide is 25000 for wild-type enzyme, 500 for mutant enzyme V154G and 2500 for mutant enzyme V154A Homo sapiens
17
-
 methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide wild-type enzyme Homo sapiens