Crystallization (Comment) | Organism |
---|---|
- |
Bacillus intermedius |
General Stability | Organism |
---|---|
Ca2+ stabilizes | Bacillus intermedius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Bacillus intermedius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
benzyloxycarbonyl-Glu-p-nitroanilide | - |
Bacillus intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Bacillus intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus intermedius | - |
strain 3-19 | - |
Bacillus intermedius Mrz 19 | - |
strain 3-19 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus intermedius |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus intermedius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Lys-Asp-Val-Tyr + H2O | - |
Bacillus intermedius | Arg-Lys-Asp + Val-Tyr | - |
? | |
Arg-Lys-Glu-Val-Tyr + H2O | completely splits Glu-Val bond, after 30 min | Bacillus intermedius | Arg-Lys-Glu + Val-Tyr | - |
? | |
benzyloxycarbonyl-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-Glu + p-nitroaniline | - |
? | |
benzyloxycarbonyl-Glu-p-nitroanilide + H2O | - |
Bacillus intermedius Mrz 19 | benzyloxycarbonyl-Glu + p-nitroaniline | - |
? | |
casein + H2O | - |
Bacillus intermedius | ? | - |
? | |
casein + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
Glucagon + H2O | - |
Bacillus intermedius | ? | - |
? | |
Glucagon + H2O | - |
Bacillus intermedius Mrz 19 | ? | - |
? | |
insulin + H2O | A-chain and B-chain | Bacillus intermedius | ? | - |
? | |
insulin + H2O | A-chain and B-chain | Bacillus intermedius Mrz 19 | ? | - |
? | |
additional information | splitting of peptide bonds of Glu and rarely of Asp in peptides and proteins | Bacillus intermedius | ? | - |
? | |
additional information | splitting of peptide bonds of Glu and rarely of Asp in peptides and proteins | Bacillus intermedius Mrz 19 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Bacillus intermedius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus intermedius |
65 | - |
in presence of Ca2+ | Bacillus intermedius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
and a second optimum at pH 9.0, hydrolysis of casein | Bacillus intermedius |
8 | - |
hydrolysis of benzyloxycarbonyl-Glu-p-nitroanilide | Bacillus intermedius |
9 | - |
and a second optimum at pH 7.5, hydrolysis of casein | Bacillus intermedius |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | 22°C, 3 h, stable | Bacillus intermedius |