Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.19 extracted from

  • Park, J.W.; Park, J.E.; Park, J.K.; Lee, J.S.
    Purification and biochemical characterization of a novel glutamyl endopeptidase secreted by a clinical isolate of Staphylococcus aureus (2011), Int. J. Mol. Med., 27, 637-645.
    View publication on PubMed
Search for more literature for 3.4.21.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
 Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.48
-
 L-Phe-L-Leu-L-Glu-4-nitroanilide pH and temperature not specified in the publication Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prothrombin + H2O Staphylococcus aureus the enzyme preferentially cleaves peptide bonds at the carboxyl sides of glutamate residues in prothrombin ?
-
 ?
prothrombin + H2O Staphylococcus aureus C-66 the enzyme preferentially cleaves peptide bonds at the carboxyl sides of glutamate residues in prothrombin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus C-66
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe-L-Leu-L-Glu-4-nitroanilide + H2O i.e. L-2135 Staphylococcus aureus L-Phe-L-Leu-L-Glu + 4-nitroaniline
-
 ?
L-Phe-L-Leu-L-Glu-4-nitroanilide + H2O i.e. L-2135 Staphylococcus aureus C-66 L-Phe-L-Leu-L-Glu + 4-nitroaniline
-
 ?
prothrombin + H2O the enzyme preferentially cleaves peptide bonds at the carboxyl sides of glutamate residues in prothrombin Staphylococcus aureus ?
-
 ?
prothrombin + H2O the enzyme preferentially cleaves peptide bonds at the carboxyl sides of glutamate residues in prothrombin Staphylococcus aureus C-66 ?
-
 ?

Synonyms

Synonyms Comment Organism
glutamate-specific serine endopeptidase
-
 Staphylococcus aureus
VSPase
-
 Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
44.4
-
 L-Phe-L-Leu-L-Glu-4-nitroanilide pH and temperature not specified in the publication Staphylococcus aureus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
 Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
 L-Phe-L-Leu-L-Glu-4-nitroanilide pH and temperature not specified in the publication Staphylococcus aureus