Protein Variants | Comment | Organism |
---|---|---|
H186T | no effect on enzyme specificity, 4.9fold increase in KM-value, 600fold decrease in kcat-value | Bacillus intermedius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide | wild-type, pH 8.5, 37°C | Bacillus intermedius | |
3.3 | - |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide | mutant H186T, pH 8.5, 37°C | Bacillus intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Bacillus intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus intermedius | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 10 months, no loss of activity for mutant H186T | Bacillus intermedius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Bacillus intermedius | ? | - |
? | |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide + H2O | - |
Bacillus intermedius | benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu + 4-nitroaniline | - |
? | |
additional information | no cleavage of certain substrates of chymotrypsin | Bacillus intermedius | ? | - |
? | |
Oxidized insulin B-chain + H2O | - |
Bacillus intermedius | ? | cleavage of bond E13-A14 | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Bacillus intermedius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
mutant H186T, 150 min, no loss of activity | Bacillus intermedius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide | mutant H186T, pH 8.5, 37°C | Bacillus intermedius | |
16.6 | - |
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide | wild-type, pH 8.5, 37°C | Bacillus intermedius |