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Literature summary for 3.4.21.12 extracted from
- Kinetic and structural characterization of mutations of glycine 216 in alpha-lytic protease: a new target for engineering substrate specificity (1995), J. Mol. Biol., 254, 720-736.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of recombinant enzymes in Escherichia coli | Lysobacter enzymogenes |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Lysobacter enzymogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G216A | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216D | no active enzyme | Lysobacter enzymogenes |
| G216F | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216G | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216H | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216I | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216K | no active enzyme | Lysobacter enzymogenes |
| G216L | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216N | no active enzyme | Lysobacter enzymogenes |
| G216P | no active enzyme | Lysobacter enzymogenes |
| G216Q | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216R | no active enzyme | Lysobacter enzymogenes |
| G216S | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216T | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216V | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216W | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
| G216Y | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Lysobacter enzymogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysobacter enzymogenes | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-Ala-Ala-Pro-X-p-nitroanilide + H2O | X: Gly, Thr, Val, Leu, Ile, Met, Phe | Lysobacter enzymogenes | ? | - |
? |  |
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