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Literature summary for 3.4.21.119 extracted from

  • Timm, D.E.
    The crystal structure of the mouse glandular kallikrein-13 (prorenin converting enzyme) (1997), Protein Sci., 6, 1418-1425.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.119

Crystallization (Commentary)

Crystallization (Comment) Organism
both hanging and sitting drop vapour diffusion method, protein solution: 57 mg/ml, precipitant solution: 10-15% PEG 8000, 200 mM Li2SO4, 100 mM sodium cacodylate, pH 6.5, room temperature, X-ray diffraction structure determination and analysis Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE Mus musculus
54000
-
 about, gel filtration and crystal structure Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Mus musculus
-
 peptides
-
 ?
Prorenin + H2O Mus musculus inactive precursor Renin + ? product is an active enzyme ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Reaction

Reaction Comment Organism Reaction ID
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin substrate binding Mus musculus
a
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin enzyme is a serine protease Mus musculus
a
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin active site is located in the cleft between two beta-barrel domains, with an ordered kallikrein loop conformation close to it Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
submandibular gland
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polypeptide + H2O
-
 Mus musculus peptides
-
 ?
Prorenin + H2O cleavage site at paired basic amino acid residues Mus musculus Renin + ?
-
 ?
Prorenin + H2O mouse prorenin from submandibular gland Mus musculus Renin + ?
-
 ?
Prorenin + H2O inactive precursor Mus musculus Renin + ? product is an active enzyme ?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE Mus musculus
More enzyme contains 2 disulfide bridges at cystines 22-157 and 136-201 Mus musculus

Synonyms

Synonyms Comment Organism
GK-13
-
 Mus musculus
glandular kallikrein 13
-
 Mus musculus
prorenin converting enzyme
-
 Mus musculus
prorenin-converting enzyme
-
 Mus musculus