Literature summary for 3.4.21.118 extracted from

Kishi, T.; Kato, M.; Shimizu, T.; Kato, K.; Matsumoto, K.; Yoshida, S.; Shiosaka, S.; Hakoshima, T.
Crystallization and preliminary X-ray analysis of neuropsin, a serine protease expressed in the limbic system of mouse brain (1997), J. Struct. Biol., 118, 248-251.

Search for more literature for 3.4.21.118

Application

Application	Comment	Organism
pharmacology	enzyme is a drug target in the treatment of epilepsy	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of proneuropsin in Spodoptera frugiperda insect cells via baculovirus infection	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, recombinant enzyme, protein solution: 24.5 mg/ml, 5 mM HEPES, pH 7.4, 100 mM NaCl, 4°C, precipitant with PEG, X-ray diffraction structure determination and analysis	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
polypeptide + H2O	Mus musculus	enzyme is implicated in various neurological processes including formation of memory	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q61955	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from SF9 insect cells	Mus musculus

Reaction

Reaction	Comment	Organism	Reaction ID
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys	enzyme is a trypsin-type serine protease	Mus musculus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
hippocampus	-	Mus musculus	-
limbic system	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polypeptide + H2O	-	Mus musculus	peptides	-	?
polypeptide + H2O	enzyme is implicated in various neurological processes including formation of memory	Mus musculus	peptides	-	?

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Release 2023.1 (January 2023)