Cloned (Comment) | Organism |
---|---|
gene KLK7, expression in Escherichia coli with secretion to the the bacterial periplasm | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme bound to specific inhibitor LEKTI domain 6, 8 mg/ml protein in sodium phosphate buffer, pH 7.5, with a reservoir solution containing 3.2 M ammonium sulfate in 0.1 M HEPES, pH 7.0, X-ray diffraction structure determination and anaylsis at 2.8 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LEKTI domain 6 | i.e. lympho-epithelial Kazaltype-related inhibitor domain 6, specific inhibition, interaction and binding structure analysis, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0159 | - |
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49862 | gene KLK7 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
epidermis | - |
Homo sapiens | - |
lung adenocarcinoma cell | - |
Homo sapiens | - |
lymphoblastic leukemia cell | - |
Homo sapiens | - |
ovary | - |
Homo sapiens | - |
pancreatic cancer cell | - |
Homo sapiens | - |
skin | kallikrein 7 is the major protease in human skin | Homo sapiens | - |
testicular carcinoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure and specific substrate site, involving conserved residues Ala190-Ser195, Val213-Cys220 and Pro225-Tyr228, molecular modeling, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
kallikrein 7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.026 | - |
- |
Homo sapiens | LEKTI domain 6 |