Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15(pREP4) cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49862 | precursor | - |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid-Sepharose column chromatography and benzamidine-Sepharose column chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
skin | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-FR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-FR + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-LR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-LR + 7-amino-4-methylcoumarin | - |
? | |
methoxy-succinyl-Arg-Pro-Tyr-p-nitroanilide + H2O | - |
Homo sapiens | methoxy-succinyl-Arg-Pro-Tyr + p-nitroaniline | - |
? | |
additional information | succinyl-Ala-Ala-Pro-Phe-tripeptidyl-p-nitroanilide is no substrate | Homo sapiens | ? | - |
? | |
additional information | the most favored P1 residue of hK7 is Tyr followed by Ala, Met, and Nle, whereas Phe is ranked rather low, and Trp seems to be totally excluded, at S2, the most preferred residue is again Tyr, well ahead of the medium-sized hydrophobic residues Leu, Nle, Thr, Met, and Phe, whereas Gly is almost not tolerated, at S3 and S4, nearly all residues are accepted with a slight preference for hydrophobic side chains | Homo sapiens | ? | - |
? | |
succinyl-AAPF-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | succinyl-AAPF + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hK7 | exhibits a chymotrypsin-like specificity preferring large hydrophobic or polar residues at the P1 position | Homo sapiens |
human stratum corneum chymotryptic enzyme | - |
Homo sapiens |