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Literature summary for 3.4.21.117 extracted from

  • Franzke, C.W.; Baici, A.; Bartels, J.; Christophers, E.; Wiedow, O.
    Antileukoprotease inhibits stratum corneum chymotryptic enzyme. Evidence for a regulative function in desquamation (1996), J. Biol. Chem., 271, 21886-21890.
    View publication on PubMed
Search for more literature for 3.4.21.117

Inhibitors

Inhibitors Comment Organism Structure
alpha1-Aantichymotrypsin 99.6% inhibition at 0.00015 mM Homo sapiens
alpha1-Macroglobulin 44.2% inhibition at 0.00015 mM Homo sapiens
Alpha1-proteinase inhibitor 89.6% inhibition at 0.00015 mM Homo sapiens
antileukoprotease 88.8% inhibition at 0.0022 mM Homo sapiens
Aprotinin 90.3% inhibition at 0.0027 mM Homo sapiens
Eglin c 99.8% inhibition at 0.00015 mM Homo sapiens
elafin 33.4% inhibition at 0.0015 mM Homo sapiens
additional information inhibition assay: 15 min preincubation of the enzyme with inhibitor at 21°C; no inhibition by Mg2+, E-64, 1,10-phenanthrolin, EDTA Homo sapiens
pepstatin A weak inhibition, 23.6% inhibition at 0.1 mM Homo sapiens
Soybean trypsin inhibitor 99.6% inhibition at 0.00015 mM Homo sapiens
Zn2+ 92.6% inhibition at 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
0.08
-
 acetyl-Phe-Val-Arg-4-nitroanilide pH 8.0, 37°C Homo sapiens
0.22
-
 succinyl-Phe-Leu-Phe-4-nitroanilide pH 8.0, 37°C Homo sapiens
0.65
-
 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide pH 8.0, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
cleavage of proteins with aromatic side chains in the P1 position enzyme is a chymotrypsin-like serine protease Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
stratum corneum plantar Homo sapiens
-
stratum corneum from heels Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Phe-Val-Arg-4-nitroanilide + H2O chromogenic substrate Homo sapiens acetyl-Phe-Val-Arg + 4-nitroaniline
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O chromogenic substrate Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O low activity Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O i.e. S2288 Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
methoxy-succinyl-Ala-Ala-Pro-Met-4-nitroanilide + H2O chromogenic substrate Homo sapiens methoxy-succinyl-Ala-Ala-Pro-Met + 4-nitroaniline
-
 ?
methoxy-succinyl-Ala-Ala-Pro-Met-4-nitroanilide + H2O low activity Homo sapiens methoxy-succinyl-Ala-Ala-Pro-Met + 4-nitroaniline
-
 ?
methoxy-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O chromogenic substrate Homo sapiens methoxy-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
methoxy-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O low activity Homo sapiens methoxy-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O best substrate Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
 ?
methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O chromogenic substrate Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
 ?
methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O i.e. S2586 Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
 ?
N-carbobenzoxy-Gly-Pro-Arg-4-nitroanilide + H2O chromogenic substrate Homo sapiens N-carbobenzoxy-Gly-Pro-Arg + 4-nitroaniline
-
 ?
N-carbobenzoxy-Gly-Pro-Arg-4-nitroanilide + H2O low activity Homo sapiens N-carbobenzoxy-Gly-Pro-Arg + 4-nitroaniline
-
 ?
N-carbobenzoxy-Val-Gly-Arg-4-nitroanilide + H2O chromogenic substrate Homo sapiens N-carbobenzoxy-Val-Gly-Arg + 4-nitroaniline
-
 ?
N-carbobenzoxy-Val-Gly-Arg-4-nitroanilide + H2O low activity Homo sapiens N-carbobenzoxy-Val-Gly-Arg + 4-nitroaniline
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
succinyl-Phe-Leu-Phe-4-nitroanilide + H2O chromogenic substrate Homo sapiens succinyl-Phe-Leu-Phe + 4-nitroaniline
-
 ?
succinyl-Phe-Leu-Phe-4-nitroanilide + H2O low activity Homo sapiens succinyl-Phe-Leu-Phe + 4-nitroaniline
-
 ?
succinyl-Val-Pro-Phe-4-nitroanilide + H2O chromogenic substrate Homo sapiens succinyl-Val-Pro-Phe + 4-nitroaniline
-
 ?
succinyl-Val-Pro-Phe-4-nitroanilide + H2O low activity Homo sapiens succinyl-Val-Pro-Phe + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
SCCE
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
0.04
-
 acetyl-Phe-Val-Arg-4-nitroanilide pH 8.0, 37°C Homo sapiens
0.05
-
 succinyl-Phe-Leu-Phe-4-nitroanilide pH 8.0, 37°C Homo sapiens
1.2
-
 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000033
-
 Eglin c pH 8.0, 37°C Homo sapiens
0.000027
-
 Aprotinin pH 8.0, 37°C Homo sapiens
0.000063
-
 antileukoprotease pH 8.0, 37°C Homo sapiens
0.0016
-
 elafin pH 8.0, 37°C Homo sapiens