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Literature summary for 3.4.21.116 extracted from

  • Hoa, N.T.; Brannigan, J.A.; Cutting, S.M.
    The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases (2002), J. Bacteriol., 184, 191-199.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D213L phenotype indistinguishable from wild type, normal spore formation, slight accumulation of the 50 kDa species Bacillus subtilis
D240L phenotype indistinguishable from wild type, normal spore formation Bacillus subtilis
D242L phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, production of an unstable protein that is rapidly cleared by secondary proteolysis Bacillus subtilis
D242N phenotype indistinguishable from wild type, normal spore formation Bacillus subtilis
D363L phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, self cleavage of enzyme appears to delayed by approximately 30 min, accumulation of the 50 kDa species Bacillus subtilis
D363N phenotype indistinguishable from wild type, normal spore formation Bacillus subtilis
H236F phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores Bacillus subtilis
H236N phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, accumulation of the 50 kDa species Bacillus subtilis
H394D production of approximately 30% fewer spores than wild type, unable to germinate properly at 37°C Bacillus subtilis
K321A phenotype indistinguishable from wild type, normal spore formation Bacillus subtilis
K387A phenotype indistinguishable from wild type, normal spore formation, slight accumulation of the 50 kDa species Bacillus subtilis
N290I production of approximately 30% fewer spores than wild type, unable to germinate properly at 37°C Bacillus subtilis
S378A phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores Bacillus subtilis
S378K phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, self cleavage of enzyme appears to delayed by approximately 30 min, accumulation of the 50 kDa species Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
endospore-forming forespore secreted by the forespore into the intermembrane space between the forespore and the mother cell membrane Bacillus subtilis 42601
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis self cleavage into at least three distinct species of 46, 45, and 44 kDa ?
-
?
additional information Bacillus subtilis PY79 self cleavage into at least three distinct species of 46, 45, and 44 kDa ?
-
?
SpoIVFA + H2O Bacillus subtilis involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing cleaved SpoIVFA + extracellular domain peptide of SpoIVFA
-
?
SpoIVFA + H2O Bacillus subtilis PY79 involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing cleaved SpoIVFA + extracellular domain peptide of SpoIVFA
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P17896 residues 187-425
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Bacillus subtilis PY79 P17896 residues 187-425
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information self cleavage into at least three distinct species of 46, 45, and 44 kDa Bacillus subtilis ?
-
?
additional information self cleavage into at least three distinct species of 46, 45, and 44 kDa Bacillus subtilis PY79 ?
-
?
SpoIVFA + H2O involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing Bacillus subtilis cleaved SpoIVFA + extracellular domain peptide of SpoIVFA
-
?
SpoIVFA + H2O involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing Bacillus subtilis PY79 cleaved SpoIVFA + extracellular domain peptide of SpoIVFA
-
?

Subunits

Subunits Comment Organism
? synthesized as 50 kDa protein at about the second h of the sporulation process, starting at h three enzyme is cleaved into at least three distinct species of 46, 45, and 44 kDa, one of which is the active form of the enzyme, further processing into enzymatically inactive 42 and 40 kDa species Bacillus subtilis

Synonyms

Synonyms Comment Organism
SpoIVB
-
Bacillus subtilis