Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D213L | phenotype indistinguishable from wild type, normal spore formation, slight accumulation of the 50 kDa species | Bacillus subtilis |
D240L | phenotype indistinguishable from wild type, normal spore formation | Bacillus subtilis |
D242L | phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, production of an unstable protein that is rapidly cleared by secondary proteolysis | Bacillus subtilis |
D242N | phenotype indistinguishable from wild type, normal spore formation | Bacillus subtilis |
D363L | phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, self cleavage of enzyme appears to delayed by approximately 30 min, accumulation of the 50 kDa species | Bacillus subtilis |
D363N | phenotype indistinguishable from wild type, normal spore formation | Bacillus subtilis |
H236F | phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores | Bacillus subtilis |
H236N | phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, accumulation of the 50 kDa species | Bacillus subtilis |
H394D | production of approximately 30% fewer spores than wild type, unable to germinate properly at 37°C | Bacillus subtilis |
K321A | phenotype indistinguishable from wild type, normal spore formation | Bacillus subtilis |
K387A | phenotype indistinguishable from wild type, normal spore formation, slight accumulation of the 50 kDa species | Bacillus subtilis |
N290I | production of approximately 30% fewer spores than wild type, unable to germinate properly at 37°C | Bacillus subtilis |
S378A | phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores | Bacillus subtilis |
S378K | phenotype indistinguishable from null mutant, fails to trigger and sigmaK signaling and to form heat and lysozyme resistant spores, self cleavage of enzyme appears to delayed by approximately 30 min, accumulation of the 50 kDa species | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endospore-forming forespore | secreted by the forespore into the intermembrane space between the forespore and the mother cell membrane | Bacillus subtilis | 42601 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | self cleavage into at least three distinct species of 46, 45, and 44 kDa | ? | - |
? | |
additional information | Bacillus subtilis PY79 | self cleavage into at least three distinct species of 46, 45, and 44 kDa | ? | - |
? | |
SpoIVFA + H2O | Bacillus subtilis | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
SpoIVFA + H2O | Bacillus subtilis PY79 | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P17896 | residues 187-425 | - |
Bacillus subtilis PY79 | P17896 | residues 187-425 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | self cleavage into at least three distinct species of 46, 45, and 44 kDa | Bacillus subtilis | ? | - |
? | |
additional information | self cleavage into at least three distinct species of 46, 45, and 44 kDa | Bacillus subtilis PY79 | ? | - |
? | |
SpoIVFA + H2O | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | Bacillus subtilis | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
SpoIVFA + H2O | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | Bacillus subtilis PY79 | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | synthesized as 50 kDa protein at about the second h of the sporulation process, starting at h three enzyme is cleaved into at least three distinct species of 46, 45, and 44 kDa, one of which is the active form of the enzyme, further processing into enzymatically inactive 42 and 40 kDa species | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
SpoIVB | - |
Bacillus subtilis |